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Severity-Related Changes of Bronchial Microbiome in Chronic Obstructive Pulmonary Disease

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Severity-Related Changes of Bronchial Microbiome in Chronic Obstructive Pulmonary Disease

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dc.contributor.author Garcia-Nuñez, Marian es_ES
dc.contributor.author Millares, Laura es_ES
dc.contributor.author Pomares, Xavier es_ES
dc.contributor.author Ferrari, Rafaela es_ES
dc.contributor.author Pérez-Brocal, Vicente es_ES
dc.contributor.author Gallego, Miguel es_ES
dc.contributor.author Espasa, Mateu es_ES
dc.contributor.author Moya, Andrés es_ES
dc.contributor.author Monsó, Eduard es_ES
dc.date.accessioned 2015-06-18T07:03:55Z
dc.date.available 2015-06-18T07:03:55Z
dc.date.issued 2014 es_ES
dc.identifier.citation Journal of Clinical Microbiology Vol. 52 Issue 12: pp. 4217-4223 es_ES
dc.identifier.uri http://hdl.handle.net/10550/44425
dc.description.abstract Bronchial colonization by potentially pathogenic microorganisms (PPMs) is often demonstrated in chronic obstructive pulmonary disease (COPD), but culture-based techniques identify only a portion of the bacteria in mucosal surfaces. The aim of the study was to determine changes in the bronchial microbiome of COPD associated with the severity of the disease. The bronchial microbiome of COPD patients was analyzed by 16S rRNA gene amplification and pyrosequencing in sputum samples obtained during stable disease. Seventeen COPD patients were studied (forced expiratory volume in the first second expressed as a percentage of the forced vital capacity [FEV1%] median, 35.0%; interquartile range [IQR], 31.5 to 52.0), providing a mean of 4,493 (standard deviation [SD], 2,598) sequences corresponding to 47 operational taxonomic units (OTUs) (SD, 17) at a 97% identity level. Patients were dichotomized according to their lung function as moderate to severe when their FEV1% values were over the median and as advanced when FEV1% values were lower. The most prevalent phyla in sputum were Proteobacteria (44%) and Firmicutes (16%), followed by Actinobacteria (13%). A greater microbial diversity was found in patients with moderate-to-severe disease, and alpha diversity showed a statistically significant decrease in patients with advanced disease when assessed by Shannon (ρ = 0.528; P = 0.029, Spearman correlation coefficient) and Chao1 (ρ = 0.53; P = 0.028, Spearman correlation coefficient) alpha-diversity indexes. The higher severity that characterizes advanced COPD is paralleled by a decrease in the diversity of the bronchial microbiome, with a loss of part of the resident flora that is replaced by a more restricted microbiota that includes PPMs. es_ES
dc.title Severity-Related Changes of Bronchial Microbiome in Chronic Obstructive Pulmonary Disease es_ES
dc.type journal article es_ES
dc.identifier.doi 10.1128/JCM.01967-14 es_ES
dc.identifier.idgrec 101954 es_ES

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