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The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

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The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

dc.contributor.author	Marcilla Díaz, Antonio	es_ES
dc.contributor.author	Garg, Gagan	es_ES
dc.contributor.author	Bernal, Dolores	es_ES
dc.contributor.author	Ranganathan, Shoba	es_ES
dc.contributor.author	Forment, Javier	es_ES
dc.contributor.author	Ortiz, Javier	es_ES
dc.contributor.author	Muñoz-Antolí, Carla	es_ES
dc.contributor.author	Dominguez, M. Victoria	es_ES
dc.contributor.author	Pedrola, Laia	es_ES
dc.contributor.author	Martinez-Blanch, Juan	es_ES
dc.contributor.author	Sotillo Gallego, Javier	es_ES
dc.contributor.author	Trelis Villanueva, María	es_ES
dc.contributor.author	Toledo Navarro, Rafael	es_ES
dc.contributor.author	Esteban Sanchis, Jose Guillermo	es_ES
dc.date.accessioned	2015-06-19T10:17:57Z
dc.date.available	2015-06-19T10:17:57Z
dc.date.issued	2012	es_ES
dc.identifier.citation	PLoS Neglected Tropical Diseases Vol. 6 Issue 2:	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44526
dc.description.abstract	BackgroundStrongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions.Principal FindingsHere we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified.ConclusionsOverall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.	es_ES
dc.title	The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1371/journal.pntd.0001513	es_ES
dc.identifier.idgrec	071614	es_ES
dc.identifier.idgrec	086224