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dc.contributor.author | Alfaro Cervelló, Clara | es_ES |
dc.contributor.author | Soriano-Navarro, Mario | es_ES |
dc.contributor.author | Mirzadeh, Zaman | es_ES |
dc.contributor.author | Alvarez-Buylla, Arturo | es_ES |
dc.contributor.author | García Verdugo, José Manuel | es_ES |
dc.date.accessioned | 2015-06-19T10:17:59Z | |
dc.date.available | 2015-06-19T10:17:59Z | |
dc.date.issued | 2012 | es_ES |
dc.identifier.citation | The Journal of comparative neurology Vol. 520 Issue 15: pp. 3528-3552 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10550/44536 | |
dc.description.abstract | Two neurogenic regions have been described in the adult brain, the lateral ventricle subventricular zone and the dentate gyrus subgranular zone. It has been suggested that neural stem cells also line the central canal of the adult spinal cord. Using transmission and scanning electron microscopy and immunostaining, we describe here the organization and cell types of the central canal epithelium in adult mice. The identity of dividing cells was determined by three-dimensional ultrastructural reconstructions of [3H]thymidine-labeled cells and confocal analysis of bromodeoxyuridine labeling. The most common cell type lining the central canal had two long motile (9+2) cilia and was vimentin+, CD24+, FoxJ1+, Sox2+ and CD133+, but nestin- and glial fibrillary acidic protein (GFAP)-. These biciliated ependymal cells of the central canal (Ecc) resembled E2 cells of the lateral ventricles, but their basal bodies were different from that of E2 or E1 cells. Interestingly, we frequently found Ecc cells with two nuclei and four cilia, suggesting they are formed by incomplete cytokinesis or cell fusion. GFAP+ astrocytes with a single cilium and an orthogonally oriented centriole were also observed. The majority of dividing cells corresponded to biciliated Ecc cells. Central canal proliferation was most common during the active period of spinal cord growth. Pairs of labeled Ecc cells were observed within the central canal in adult mice 2.5 weeks post-labeling. Our work suggests that the vast majority of postnatal dividing cells in the central canal are Ecc cells and their proliferation is associated with the growth of the spinal cord. | es_ES |
dc.subject | central canal | es_ES |
dc.subject | ultrastructure | es_ES |
dc.subject | ependyma | es_ES |
dc.subject | cilia | es_ES |
dc.title | Biciliated ependymal cell proliferation contributes to spinal cord growth | es_ES |
dc.type | journal article | es_ES |
dc.identifier.doi | 10.1002/cne.23104 | es_ES |
dc.identifier.idgrec | 076903 | es_ES |