NAGIOS: RODERIC FUNCIONANDO

Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated chondrosarcomas of bone

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated chondrosarcomas of bone

dc.contributor.author	van Oosterwijk, Jolieke G.	es_ES
dc.contributor.author	de Jong, Danielle	es_ES
dc.contributor.author	van Ruler, Maayke AJH	es_ES
dc.contributor.author	Hogendoorn, Pancras C. W.	es_ES
dc.contributor.author	Dijkstra, PD Sander	es_ES
dc.contributor.author	van Rijswijk, Carla SP	es_ES
dc.contributor.author	Machado, Isidro	es_ES
dc.contributor.author	Llombart Bosch, Antonio	es_ES
dc.contributor.author	Szuhai, Karoly	es_ES
dc.contributor.author	Bovée, Judith VMG	es_ES
dc.date.accessioned	2015-06-19T10:17:59Z
dc.date.available	2015-06-19T10:17:59Z
dc.date.issued	2012	es_ES
dc.identifier.citation	BMC Cancer Vol. 12 pp. 375-375	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44542
dc.description.abstract	BackgroundChondrosarcoma is the second most common primary sarcoma of bone. High-grade conventional chondrosarcoma and dedifferentiated chondrosarcoma have a poor outcome. In pre-clinical research aiming at the identification of novel treatment targets, the need for representative cell lines and model systems is high, but availability is scarce.MethodsWe developed and characterized three cell lines, derived from conventional grade III chondrosarcoma (L835), and dedifferentiated chondrosarcoma (L2975 and L3252) of bone. Proliferation and migration were studied and we used COBRA-FISH and array-CGH for karyotyping and genotyping. Immunohistochemistry for p16 and p53 was performed as well as TP53 and IDH mutation analysis. Cells were injected into nude mice to establish their tumorigenic potential.ResultsWe show that the three cell lines have distinct migrative properties, L2975 had the highest migration rate and showed tumorigenic potential in mice. All cell lines showed chromosomal rearrangements with complex karyotypes and genotypic aberrations were conserved throughout late passaging of the cell lines. All cell lines showed loss of CDKN2A, while TP53 was wild type for exons 5–8. L835 has an IDH1 R132C mutation, L2975 an IDH2 R172W mutation and L3252 is IDH wild type.ConclusionsBased on the stable culturing properties of these cell lines and their genotypic profile resembling the original tumors, these cell lines should provide useful functional models to further characterize chondrosarcoma and to evaluate new treatment strategies.	es_ES
dc.subject	Bone neoplasm	es_ES
dc.subject	Chondrosarcoma	es_ES
dc.subject	Cell line	es_ES
dc.subject	IDH1	es_ES
dc.subject	IDH2	es_ES
dc.subject	p16	es_ES
dc.title	Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated chondrosarcomas of bone	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1186/1471-2407-12-375	es_ES
dc.identifier.idgrec	079936	es_ES