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Targeted profiling of circulating and hepatic bile acids in human, mouse, and rat using a UPLC-MRM-MS-validated method[S]

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Targeted profiling of circulating and hepatic bile acids in human, mouse, and rat using a UPLC-MRM-MS-validated method[S]

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dc.contributor.author García Cañaveras, Juan Carlos es_ES
dc.contributor.author Donato Martín, María Teresa es_ES
dc.contributor.author Castell Ripoll, José Vicente es_ES
dc.contributor.author Lahoz Rodríguez, Agustín es_ES
dc.date.accessioned 2015-06-19T10:18:00Z
dc.date.available 2015-06-19T10:18:00Z
dc.date.issued 2012 es_ES
dc.identifier.citation Journal of Lipid Research Vol. 53 Issue 10: pp. 2231-2241 es_ES
dc.identifier.uri http://hdl.handle.net/10550/44548
dc.description.abstract Bile acids (BAs) are a group of chemically related steroids recognized as regulatory molecules whose profiles can change in different physio-pathological situations. We have developed a sensitive, fast, and reproducible ultraperformance liquid chromatography/multiple reaction monitoring/mass spectrometry method to determine the tissue and sera BA profiles in different species (human, rat, and mouse) by quantifying 31 major and minor BA species in a single 21-min run. The method has been validated according to FDA guidelines, and it generally provides good results in terms of intra- and interday precision (less than 8.6% and 16.0%, respectively), accuracy (relative error measurement between –11.9% and 8.6%), and linearity (R2 > 0.996 and dynamic ranges between two and four orders of magnitude), with limits of quantification between 2.5 and 20 nM. The new analytical approach was applied to determine BA concentrations in human, rat, and mouse serum and in liver tissue. Our comparative study confirmed and extended previous reports, showing marked interspecies differences in circulating and hepatic BA composition. The targeted analysis revealed the presence of unexpected minoritary BAs, such as tauro-alpha-Muricholic acid in human serum, thus allowing us to obtain a thorough profiling of human samples. Its great sensitivity, low sample requirements (25 µl of serum, 5 mg of tissue), and comprehensive capacity to profile a considerable number of BAs make the present method a good choice to study BA metabolism in physiological and pathological situations, particularly in toxicological studies. es_ES
dc.subject lipidomics es_ES
dc.subject metabolomics es_ES
dc.subject targeted analysis es_ES
dc.title Targeted profiling of circulating and hepatic bile acids in human, mouse, and rat using a UPLC-MRM-MS-validated method[S] es_ES
dc.type journal article es_ES
dc.identifier.doi 10.1194/jlr.D028803 es_ES
dc.identifier.idgrec 082675 es_ES

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