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Amplification, contraction and genomic spread of a satellite DNA family (E180) in Medicago (Fabaceae) and allied genera

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Amplification, contraction and genomic spread of a satellite DNA family (E180) in Medicago (Fabaceae) and allied genera

dc.contributor.author	Rosato, Marcela	es_ES
dc.contributor.author	Galián, José A.	es_ES
dc.contributor.author	Rosselló Picornell, Josep Antoni	es_ES
dc.date.accessioned	2015-06-19T10:21:23Z
dc.date.available	2015-06-19T10:21:23Z
dc.date.issued	2012	es_ES
dc.date.issued	2011	es_ES
dc.identifier.citation	Annals of Botany Vol. 109 Issue 4: pp. 773-782	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44577
dc.description.abstract	Background and AimsSatellite DNA is a genomic component present in virtually all eukaryotic organisms. The turnover of highly repetitive satellite DNA is an important element in genome organization and evolution in plants. Here we assess the presence and physical distribution of the repetitive DNA E180 family in Medicago and allied genera. Our goals were to gain insight into the karyotype evolution of Medicago using satellite DNA markers, and to evaluate the taxonomic and phylogenetic signal of a satellite DNA family in a genus hypothesized to have a complex evolutionary history.MethodsSeventy accessions from Medicago, Trigonella, Melilotus and Trifolium were analysed by PCR to assess the presence of the repetitive E180 family, and fluorescence in situ hybridization (FISH) was used for physical mapping in somatic chromosomes.Key ResultsThe E180 repeat unit was PCR-amplified in 37 of 40 taxa in Medicago, eight of 12 species of Trigonella, six of seven species of Melilotus and in two of 11 Trifolium species. Examination of the mitotic chromosomes revealed that only 13 Medicago and two Trigonella species showed FISH signals using the E180 probe. Stronger hybridization signals were observed in subtelomeric and interstitial loci than in the pericentromeric loci, suggesting this satellite family has a preferential genomic location. Not all 13 Medicago species that showed FISH localization of the E180 repeat were phylogenetically related. However, nine of these species belong to the phylogenetically derived clade including the M. sativa and M. arborea complexes.ConclusionsThe use of the E180 family as a phylogenetic marker in Medicago should be viewed with caution. Its amplification appears to have been produced through recurrent and independent evolutionary episodes in both annual and perennial Medicago species as well as in basal and derived clades.	es_ES
dc.subject	Medicago	es_ES
dc.subject	Trigonella	es_ES
dc.subject	Melilotus	es_ES
dc.subject	Trifolium	es_ES
dc.subject	satellite DNA	es_ES
dc.subject	repetitive E180 family	es_ES
dc.subject	FISH	es_ES
dc.title	Amplification, contraction and genomic spread of a satellite DNA family (E180) in Medicago (Fabaceae) and allied genera	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1093/aob/mcr309	es_ES
dc.identifier.idgrec	089510	es_ES