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mRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts

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mRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts

dc.contributor.author	Kuijjer, Marieke L	es_ES
dc.contributor.author	Namløs, Heidi M	es_ES
dc.contributor.author	Hauben, Esther I	es_ES
dc.contributor.author	Machado, Isidro	es_ES
dc.contributor.author	Kresse, Stine H	es_ES
dc.contributor.author	Serra, Massimo	es_ES
dc.contributor.author	Llombart Bosch, Antonio	es_ES
dc.contributor.author	Hogendoorn, Pancras C. W.	es_ES
dc.contributor.author	Meza-Zepeda, Leonardo A	es_ES
dc.contributor.author	Myklebost, Ola	es_ES
dc.contributor.author	Cleton-Jansen, Anne-Marie	es_ES
dc.date.accessioned	2015-06-22T09:47:26Z
dc.date.available	2015-06-22T09:47:26Z
dc.date.issued	2011	es_ES
dc.identifier.citation	BMC Medical Genomics Vol. 4 pp. 66-66	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44654
dc.description.abstract	BackgroundConventional high-grade osteosarcoma is a primary malignant bone tumor, which is most prevalent in adolescence. Survival rates of osteosarcoma patients have not improved significantly in the last 25 years. Aiming to increase this survival rate, a variety of model systems are used to study osteosarcomagenesis and to test new therapeutic agents. Such model systems are typically generated from an osteosarcoma primary tumor, but undergo many changes due to culturing or interactions with a different host species, which may result in differences in gene expression between primary tumor cells, and tumor cells from the model system. We aimed to investigate whether gene expression profiles of osteosarcoma cell lines and xenografts are still comparable to those of the primary tumor.MethodsWe performed genome-wide mRNA expression profiling on osteosarcoma biopsies (n = 76), cell lines (n = 13), and xenografts (n = 18). Osteosarcoma can be subdivided into several histological subtypes, of which osteoblastic, chondroblastic, and fibroblastic osteosarcoma are the most frequent ones. Using nearest shrunken centroids classification, we generated an expression signature that can predict the histological subtype of osteosarcoma biopsies.ResultsThe expression signature, which consisted of 24 probes encoding for 22 genes, predicted the histological subtype of osteosarcoma biopsies with a misclassification error of 15%. Histological subtypes of the two osteosarcoma model systems, i.e. osteosarcoma cell lines and xenografts, were predicted with similar misclassification error rates (15% and 11%, respectively).ConclusionsBased on the preservation of mRNA expression profiles that are characteristic for the histological subtype we propose that these model systems are representative for the primary tumor from which they are derived.	es_ES
dc.title	mRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1186/1755-8794-4-66	es_ES
dc.identifier.idgrec	068446	es_ES