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A Genomic Reappraisal of Symbiotic Function in the Aphid/Buchnera Symbiosis: Reduced Transporter Sets and Variable Membrane Organisations

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A Genomic Reappraisal of Symbiotic Function in the Aphid/Buchnera Symbiosis: Reduced Transporter Sets and Variable Membrane Organisations

dc.contributor.author	Charles, Hubert	es_ES
dc.contributor.author	Balmand, Séverine	es_ES
dc.contributor.author	Lamelas, Araceli	es_ES
dc.contributor.author	Cottret, Ludovic	es_ES
dc.contributor.author	Pérez-Brocal, Vicente	es_ES
dc.contributor.author	Burdin, Béatrice	es_ES
dc.contributor.author	Latorre, Amparo	es_ES
dc.contributor.author	Febvay, Gérard	es_ES
dc.contributor.author	Colella, Stefano	es_ES
dc.contributor.author	Calevro, Federica	es_ES
dc.contributor.author	Rahbé, Yvan	es_ES
dc.date.accessioned	2015-06-22T09:50:05Z
dc.date.available	2015-06-22T09:50:05Z
dc.date.issued	2011	es_ES
dc.identifier.citation	PLoS ONE Vol. 6 Issue 12:	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44661
dc.description.abstract	Buchnera aphidicola is an obligate symbiotic bacterium that sustains the physiology of aphids by complementing their exclusive phloem sap diet. In this study, we reappraised the transport function of different Buchnera strains, from the aphids Acyrthosiphon pisum, Schizaphis graminum, Baizongia pistaciae and Cinara cedri, using the re-annotation of their transmembrane proteins coupled with an exploration of their metabolic networks. Although metabolic analyses revealed high interdependencies between the host and the bacteria, we demonstrate here that transport in Buchnera is assured by low transporter diversity, when compared to free-living bacteria, being mostly based on a few general transporters, some of which probably have lost their substrate specificity. Moreover, in the four strains studied, an astonishing lack of inner-membrane importers was observed. In Buchnera, the transport function has been shaped by the distinct selective constraints occurring in the Aphididae lineages. Buchnera from A. pisum and S. graminum have a three-membraned system and similar sets of transporters corresponding to most compound classes. Transmission electronic microscopic observations and confocal microscopic analysis of intracellular pH fields revealed that Buchnera does not show any of the typical structures and properties observed in integrated organelles. Buchnera from B. pistaciae seem to possess a unique double membrane system and has, accordingly, lost all of its outer-membrane integral proteins. Lastly, Buchnera from C. cedri revealed an extremely poor repertoire of transporters, with almost no ATP-driven active transport left, despite the clear persistence of the ancestral three-membraned system.	es_ES
dc.title	A Genomic Reappraisal of Symbiotic Function in the Aphid/Buchnera Symbiosis: Reduced Transporter Sets and Variable Membrane Organisations	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1371/journal.pone.0029096	es_ES
dc.identifier.idgrec	070263	es_ES
dc.identifier.idgrec	080894