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Control of Cell Migration and Inflammatory Mediators Production by CORM-2 in Osteoarthritic Synoviocytes

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Control of Cell Migration and Inflammatory Mediators Production by CORM-2 in Osteoarthritic Synoviocytes

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dc.contributor.author García-Arnandis, Isabel es_ES
dc.contributor.author Guillén Salazar, María Isabel es_ES
dc.contributor.author Gomar Sancho, Francisco es_ES
dc.contributor.author Castejón, Miguel Angel es_ES
dc.contributor.author Alcaraz Tormo, María José es_ES
dc.date.accessioned 2015-06-22T09:52:41Z
dc.date.available 2015-06-22T09:52:41Z
dc.date.issued 2011 es_ES
dc.identifier.citation PLoS ONE Vol. 6 Issue 9: es_ES
dc.identifier.uri http://hdl.handle.net/10550/44682
dc.description.abstract BackgroundOsteoarthritis (OA) is the most widespread degenerative joint disease. Inflamed synovial cells contribute to the release of inflammatory and catabolic mediators during OA leading to destruction of articular tissues. We have shown previously that CO-releasing molecules exert anti-inflammatory effects in animal models and OA chondrocytes. We have studied the ability of CORM-2 to modify the migration of human OA synoviocytes and the production of chemokines and other mediators sustaining inflammatory and catabolic processes in the OA joint.Methodology/Principal FindingsOA synoviocytes were stimulated with interleukin(IL)-1β in the absence or presence of CORM-2. Migration assay was performed using transwell chambers. Gene expression was analyzed by quantitative PCR and protein expression by Western Blot and ELISA. CORM-2 reduced the proliferation and migration of OA synoviocytes, the expression of IL-8, CCL2, CCL20, matrix metalloproteinase(MMP)-1 and MMP-3, and the production of oxidative stress. We found that CORM-2 reduced the phosphorylation of extracellular signal-regulated kinase1/2, c-Jun N-terminal kinase1/2 and to a lesser extent p38. Our results also showed that CORM-2 significantly decreased the activation of nuclear factor-κB and activator protein-1 regulating the transcription of chemokines and MMPs in OA synoviocytes.Conclusion/SignificanceA number of synoviocyte functions relevant in OA synovitis and articular degradation can be down-regulated by CORM-2. These results support the interest of this class of agents for the development of novel therapeutic strategies in inflammatory and degenerative conditions. es_ES
dc.title Control of Cell Migration and Inflammatory Mediators Production by CORM-2 in Osteoarthritic Synoviocytes es_ES
dc.type journal article es_ES
dc.identifier.doi 10.1371/journal.pone.0024591 es_ES
dc.identifier.idgrec 076646 es_ES

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