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Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines

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Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines

dc.contributor.author	Ortiz, Cristian M	es_ES
dc.contributor.author	Ito, Tetsuo	es_ES
dc.contributor.author	Hashimoto, Yosuke	es_ES
dc.contributor.author	Nagayama, Satoshi	es_ES
dc.contributor.author	Iwai, Akira	es_ES
dc.contributor.author	Tsunoda, Shigeru	es_ES
dc.contributor.author	Sato, Fumiaki	es_ES
dc.contributor.author	Martorell, Miguel	es_ES
dc.contributor.author	Garcia, Jose Angel	es_ES
dc.contributor.author	Perez, Ana	es_ES
dc.contributor.author	Shimada, Yutaka	es_ES
dc.date.accessioned	2015-06-29T10:32:27Z
dc.date.available	2015-06-29T10:32:27Z
dc.date.issued	2010	es_ES
dc.identifier.citation	Diagnostic Pathology Vol. 5 pp. 41-41	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44796
dc.description.abstract	BackgroundFascin induces membrane protrusions and cell motility. Fascin overexpression was associated with poor prognosis, and its downregulation reduces cell motility and invasiveness in esophageal squamous cell carcinoma (ESCC). Using a stable knockdown cell line, we revealed the effect of fascin on cell growth, cell adhesion and tumor formation.MethodsWe examined whether fascin is a potential target in ESCC using in vitro and in vivo studies utilizing a specific siRNA. We established a stable transfectant with downregulated fascin from KYSE170 cell line.ResultsThe fascin downregulated cell lines showed a slower growth pattern by 40.3% (p < 0.01) and detachment from collagen-coated plates by 53.6% (p < 0.01), compared to mock cells, suggesting that fascin plays a role in cell growth by maintaining cell adhesion to the extracellular matrix. In vivo, the tumor size was significantly smaller in the tumor with fascin knockdown cells than in mock cells by 95% at 30 days after inoculation.ConclusionsThese findings suggest that fascin overexpression plays a role in tumor growth and progression in ESCC and that cell death caused by its downregulation might be induced by cell adhesion loss. This indicates that targeting fascin pathway could be a novel therapeutic strategy for the human ESCC.	es_ES
dc.title	Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1186/1746-1596-5-41	es_ES
dc.identifier.idgrec	063073	es_ES