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Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species

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Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species

dc.contributor.author	Hernández Rodríguez, Carmen Sara
dc.contributor.author	Hernández Martínez, Patricia
dc.contributor.author	Van Rie, Jeroen
dc.contributor.author	Ferré Manzanero, Juan
dc.date.accessioned	2015-12-11T13:03:29Z
dc.date.available	2015-12-11T13:03:29Z
dc.date.issued	2012
dc.identifier.citation	Hernández Rodríguez, Carmen Sara Hernández Martínez, Patricia J. Van Rie Van Rie, Jeroen Ferré Manzanero, Juan 2012 Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species Applied and Environmental Microbiology 78 11 4048 4050
dc.identifier.uri	http://hdl.handle.net/10550/49196
dc.description.abstract	Cry1Fa insecticidal protein was successfully radiolabeled with 125I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (Kd [dissociation constant] and Rt [concentration of binding sites]) for these six insect species.
dc.language.iso	eng
dc.relation.ispartof	Applied and Environmental Microbiology, 2012, vol. 78, num. 11, p. 4048-4050
dc.subject	Plaguicides
dc.subject	Proteïnes
dc.subject	Bioquímica
dc.subject	Biotecnologia agrícola
dc.subject	Plagues Control
dc.title	Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species
dc.type	journal article	es_ES
dc.date.updated	2015-12-11T13:03:29Z
dc.identifier.doi	10.1128/AEM.07591-11
dc.identifier.idgrec	085726
dc.rights.accessRights	open access	es_ES