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Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion

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Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion

dc.contributor.author	Yang, Guowei
dc.contributor.author	Hernández Rodríguez, Carmen Sara
dc.contributor.author	Beeton, Michael L.
dc.contributor.author	Wilkinson, Paul
dc.contributor.author	Ffrench-Constant, Richard H.
dc.contributor.author	Waterfield, Nicholas R.
dc.date.accessioned	2015-12-15T11:56:11Z
dc.date.available	2015-12-15T11:56:11Z
dc.date.issued	2012
dc.identifier.citation	Yang, Guowei Hernández Rodríguez, Carmen Sara Beeton, Michael L. Wilkinson, Paul Ffrench-Constant, Richard H. Waterfield, Nicholas R. 2012 Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion Plos Pathogens 8 5 e1002692-1 e1002692-12
dc.identifier.uri	http://hdl.handle.net/10550/49264
dc.description.abstract	The Toxin Complex (TC) is a large multi-subunit toxin first characterized in the insect pathogens Photorhabdus and Xenorhabdus , but now seen in a range of pathogens, including those of humans. These complexes comprise three protein subunits, A, B and C which in the Xenorhabdus toxin are found in a 4:1:1 stoichiometry. Some TCs have been demonstrated to exhibit oral toxicity to insects and have the potential to be developed as a pest control technology. The lack of recognisable signal sequences in the three large component proteins hinders an understanding of heir mode of secretion. Nevertheless, we have shown the Photorhabdus luminescens (Pl) Tcd complex has been shown to associate with the bacteria's surface, although some strains can also release it into the surrounding milieu. The large number of tc gene homologues in Pl make study of the export process difficult and as such we have developed and validated a heterologous Escherichia coli expression model to study the release of these important toxins. In addition to this model, we have used comparative genomics between a strain that releases high levels of Tcd into the supernatant and one that retains the toxin on its surface, to identify a protein responsible for enhancing secretion and release of these toxins. This protein is a putative lipase (Pdl1) which is regulated by a small tightly linked antagonist protein (Orf53). The identification of homologues of these in other bacteria, linked to other virulence factor operons, such as type VI secretion systems, suggests that these genes represent a general and widespread mechanism for enhancing toxin release in Gram negative pathogens.
dc.language.iso	eng
dc.relation.ispartof	Plos Pathogens, 2012, vol. 8, num. 5, p. e1002692-1-e1002692-12
dc.subject	Proteïnes
dc.subject	Bacteris patògens
dc.subject	Microbiologia
dc.title	Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion
dc.type	journal article	es_ES
dc.date.updated	2015-12-15T11:56:12Z
dc.identifier.doi	10.1371/journal.ppat.1002692
dc.identifier.idgrec	104265
dc.rights.accessRights	open access	es_ES