NAGIOS: RODERIC FUNCIONANDO

Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.

dc.contributor.author	Gómez Navarro, Natalia
dc.contributor.author	Jordán Plá, Antonio
dc.contributor.author	Estruch Ros, Francisco
dc.contributor.author	Pérez Ortín, José Enrique
dc.date.accessioned	2016-03-07T11:18:56Z
dc.date.available	2016-03-07T11:18:56Z
dc.date.issued	2016
dc.identifier.citation	Gómez Navarro, Natalia Jordán Plá, Antonio Estruch Ros, Francisco Pérez Ortín, José Enrique 2016 Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast. Bmc Genomics 17 183-1 183-9
dc.identifier.uri	http://hdl.handle.net/10550/51733
dc.description.abstract	The formation of the pre-initiation complex in eukaryotic genes is a key step in transcription initiation. The TATA-binding protein (TBP) is a universal component of all pre-initiation complexes for all kinds of RNA polymerase II (RNA pol II) genes, including those with a TATA or a TATA-like element, both those that encode proteins and those that transcribe non-coding RNAs. Mot1 and the negative cofactor 2 (NC2) complex are regulators of TBP, and it has been shown that depletion of these factors in yeast leads to defects in the control of transcription initiation that alter cryptic transcription levels in selected yeast loci. Results In order to cast light on the molecular functions of NC2, we performed genome-wide studies in conditional mutants in yeast NC2 essential subunits Ydr1 and Bur6. Our analyses show a generally increased level of cryptic transcription in all kinds of genes upon depletion of NC2 subunits, and that each kind of gene (canonical or ncRNAs, TATA or TATA-like) shows some differences in the cryptic transcription pattern for each NC2 mutant. Conclusions We conclude that NC2 plays a general role in transcription initiation in RNA polymerase II genes that is related with its known TBP interchange function from free to promoter bound states. Therefore, loss of the NC2 function provokes increases in cryptic transcription throughout the yeast genome. Our results also suggest functional differences between NC2 subunits Ydr1 and Bur6.
dc.language.iso	eng
dc.relation.ispartof	Bmc Genomics, 2016, vol. 17, p. 183-1-183-9
dc.subject	Genètica molecular
dc.subject	Transcripció genètica
dc.subject	Saccharomyces
dc.subject	Proteïnes
dc.title	Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.
dc.type	journal article	es_ES
dc.date.updated	2016-03-07T11:18:56Z
dc.identifier.doi	10.1186/s12864-016-2536-2
dc.identifier.idgrec	109843
dc.rights.accessRights	open access	es_ES