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Perlecan Maintains microvessel integrity in vivo and modulates their formation in vitro

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Perlecan Maintains microvessel integrity in vivo and modulates their formation in vitro

dc.contributor.author	Gustafsson, E.
dc.contributor.author	Almonte-Becerril, M.
dc.contributor.author	Bloch, W.
dc.contributor.author	Costell Rosselló, Mercedes
dc.date.accessioned	2016-06-08T13:40:49Z
dc.date.available	2016-06-08T13:40:49Z
dc.date.issued	2013
dc.identifier.citation	Gustafsson, E. Almonte-Becerril, M. Bloch, W. Costell Rosselló, Mercedes 2013 Perlecan Maintains microvessel integrity in vivo and modulates their formation in vitro Plos One 8 1 e53715
dc.identifier.uri	http://hdl.handle.net/10550/53992
dc.description.abstract	Perlecan is a heparan sulfate proteoglycan assembled into the vascular basement membranes (BMs) during vasculogenesis. In the present study we have investigated vessel formation in mice, teratomas and embryoid bodies (EBs) in the absence of perlecan. We found that perlecan was dispensable for blood vessel formation and maturation until embryonic day (E) 12.5. At later stages of development 40% of mutant embryos showed dilated microvessels in brain and skin, which ruptured and led to severe bleedings. Surprisingly, teratomas derived from perlecan-null ES cells showed efficient contribution of perlecan-deficient endothelial cells to an apparently normal tumor vasculature. However, in perlecan-deficient EBs the area occupied by an endothelial network and the number of vessel branches were significantly diminished. Addition of FGF-2 but not VEGF165 rescued the in vitro deficiency of the mutant ES cells. Furthermore, in the absence of perlecan in the EB matrix lower levels of FGFs are bound, stored and available for cell surface presentation. Altogether these findings suggest that perlecan supports the maintenance of brain and skin subendothelial BMs and promotes vasculo- and angiogenesis by modulating FGF-2 function.
dc.language.iso	eng
dc.relation.ispartof	Plos One, 2013, vol. 8, num. 1, p. e53715
dc.subject	Biotecnologia
dc.subject	Proteïnes de membrana
dc.subject	Cèl·lules
dc.title	Perlecan Maintains microvessel integrity in vivo and modulates their formation in vitro
dc.type	journal article	es_ES
dc.date.updated	2016-06-08T13:40:49Z
dc.identifier.doi	10.1371/journal.pone.0053715
dc.identifier.idgrec	085840
dc.rights.accessRights	open access	es_ES