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Effect of the methacrylate-based endodontic sealer Epiphany on rat peritoneal macrophages viability

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Effect of the methacrylate-based endodontic sealer Epiphany on rat peritoneal macrophages viability

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dc.contributor.author Martín González, Jenifer es
dc.contributor.author Castellanos Cosano, Lizett es
dc.contributor.author López Frías, Francisco Javier es
dc.contributor.author Sánchez Domínguez, Benito es
dc.contributor.author Calvo Monroy, Cristina es
dc.contributor.author Alonso Ezpeleta, Luis Oscar es
dc.contributor.author Segura-Egea, Juan J. es
dc.date.accessioned 2016-06-20T07:13:25Z
dc.date.available 2016-06-20T07:13:25Z
dc.date.issued 2011 es
dc.identifier.citation Martín González, Jenifer ; Castellanos Cosano, Lizett ; López Frias, Javier ; Sánchez Domínguez, Benito ; Calvo Monroy, Cristina ; Alonso Ezpeleta, Luis Oscar ; Segura-Egea, Juan J.. Effect of the methacrylate-based endodontic sealer Epiphany on rat peritoneal macrophages viability. En: Journal of Clinical and Experimental Dentistry, 2011, Vol. 3, No. 3: 216-221 es
dc.identifier.uri http://hdl.handle.net/10550/54119
dc.description.abstract Objective: To evaluate the effect of the endodontic sealer Epiphany on rat peritoneal macrophages viability. Materials and methods: Peritoneal macrophages were obtained from Wistar rats and resuspended in RPMI- 1640 medium. Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of Epiphany, AH 26 and AH Plus sealers on RPMI-1640 medium were tested for cytotoxicity to rat peritoneal macrophages using the trypan blue dye exclusion assay. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. Results: Crude extract of Epiphany killed 51% of cells, but was less cytotoxic that crude extracts of AH Plus and AH 26, which killed 81% and 86% of cells, respectively. Ten-fold dilutions of Epiphany, AH Plus and AH 26 killed 44%, 56%, 62% of macrophages, respectively. A hundred dilution of Epiphany only killed 7% of macrophages, but the same dilution of AH Plus and AH 26 killed 10% and 31% of macrophages, respectively. Lower dilutions of sealer extracts caused minimal cell death as compared to the control groups (p>0.05). Conclusions: The methacrylate-based endodontic sealer Epiphany showed lower cytotoxicity on macrophages than resin-based sealers AH Plus and AH 26. Dilution of elutes of the three materials by tenfold markedly reduced their effects en_US
dc.subject Odontología es
dc.subject Ciencias de la salud es
dc.title Effect of the methacrylate-based endodontic sealer Epiphany on rat peritoneal macrophages viability es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.type.hasVersion VoR es_ES

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