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Growth factors reléase on P-PRP activeted with trombin assessment assay

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Growth factors reléase on P-PRP activeted with trombin assessment assay

dc.contributor.author	Carrasco Luna, Joaquín
dc.contributor.author	Bonete Lluch, D.
dc.contributor.author	Silvestre Muñoz, Antonio
dc.contributor.author	Gomar Sancho, Francisco
dc.date.accessioned	2016-06-24T11:53:42Z
dc.date.available	2016-06-24T11:53:42Z
dc.date.issued	2015
dc.identifier.citation	Carrasco Luna, Joaquin; Bonete, Daniel; Silvestre Muñoz, Antonio; Gomar Sancho, Francisco (2015) Growth factors reléase on P-PRP activeted with trombin assessment assay American international journal of biology 3 1 33 48
dc.identifier.uri	http://hdl.handle.net/10550/54194
dc.description.abstract	The development of platelet concentrates has generated great expectations in bone surgery. These compounds provides autologous growth factors for tissue healing and regeneration, but disparity of methods hinders the patterns of use. The objective of our study is proposing a standard model for Platelet Rich Plasma preparation, involving growth factors release. Sheep peripheral blood were obtained with anticoagulant citrate dextrose 50 N (Citra), three different speed centrifugation methods and plasma activation (1000 units of thrombin and 10% CaCl2 per ml were performed. The release of growth factors were determined by ELISA (R & D systems). The higher platelets concentration was obtained with two centrifugations, first 500 g 10 min, followed by 1900g for 8 min. After activation clotting immediate release of growth factors by platelet degranulation were achieved. The PDGF increased more than 1.9 fold till 537.9 pg/ml and TGF-β1 increases almost 8 times till 7150 pgr/ml in 180 min. Our standard method proposed, triggers the release of growth factors, immediately after activation with a dose-response relationship between platelets and growth factors. This means that the PRP prepared in our experimental conditions is really a plasma rich in growth factors ready for clinical use.
dc.language.iso	eng
dc.relation.ispartof	American international journal of biology, 2015, vol. 3, num. 1, p. 33-48
dc.subject	Sang
dc.subject	Ossos
dc.subject	Cirurgia
dc.subject	Traumatologia
dc.title	Growth factors reléase on P-PRP activeted with trombin assessment assay
dc.type	journal article	es_ES
dc.date.updated	2016-06-24T11:53:42Z
dc.identifier.doi	10.15640/aijb.v3n1a3
dc.identifier.idgrec	111212
dc.rights.accessRights	open access	es_ES