Objectives: A dentist is required to detect and recognise oral lesions and inform the patient accordingly by providing a diagnosis and adequate treatment plan. Biopsy serves as an important aid in achieving this goal; however, its use is not so widespread in general dental practice. The objective of the present study was to explore the attitudes of general dental practitioners in Belgaum city towards biopsy for diagnosis of oral lesions. Material and methods: A self designed questionnaire was administered to 74 general dental practitioners in Belgaum city, in the southern region of India, consisting of several items addressing the socio-demographic and professional aspects and their attitudes towards oral biopsy procedures. Results: The response rate was exceptionally high i.e. 90.54%. All the dentists felt that biopsy was an important tool in diagnosis of oral lesions but many still did not venture to undertake it on their own and preferred referring it to a specialist or higher care centre. This was mainly due to lack of experience and patient factors. There were also conflicting results regarding referral diagnostic pathology services and preservation of the biopsy specimens. Conclusion: This emphasizes the need for higher levels of importance to be placed on this aspect in undergraduate and postgraduate dental curriculum. Organisation of specific training or continuing dental education programmes to enhance their practical skills could aid in increasing the utility of this important tool in diagnosis of oral lesions