Introduction: The combination of carbamide peroxide, thiocyanate or enzymes such as amynoglucosidase or gluco-oxidase is able to reduce gingival inflammation. Objective: To evaluate antiseptic efficacy, in relation to reduced gingival bleeding, of a new dentifrice containing carbamide peroxide and lactorperoxidase. Materials and method: A sample of 32 patients, with periodontal pockets of more than 3mm in the vestibular area and 4 mm at interproximal sites, was divided into two groups of 16 and 17 patients respectively. Group A used a dentifrice containing 3% carbamide peroxide and 5% lactoperoxidase plus 0.022% sodium fluoride for three weeks followed by a control dentifrice with only 0.022% sodium fluoride for another three weeks. Group B used the same dentifrices but in reverse order. Efficacy was assessed by measuring the bleeding sites at the start of the study, and after application of the first and second dentifrices. Results: After applying the toothpaste object of this study, group A showed a significant reduction in bleeding sites (p<0.05) and a slight non-significant increase when the control toothpaste was applied (p>0.05). After application of the control toothpaste, group B showed a significant reduction in the average number of bleeding sites and after application of the study toothpaste the group showed a greater reduction in the average number of bleeding sites, which was also significant in relation to the first phase (p<0.05). Conclusions: Reduction of gingival bleeding by the carbamide peroxide based dentifrice with lactoperoxidase was found to be superior to the control fluoride dentifrice.