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Effects of ranolazine on astrocytes and neurons in primary culture

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Effects of ranolazine on astrocytes and neurons in primary culture

dc.contributor.author	Aldasoro Celaya, Martín
dc.contributor.author	Guerra Ojeda, Sol
dc.contributor.author	Aguirre Rueda, Diana
dc.contributor.author	Mauricio, Maria Dolores
dc.contributor.author	Vila Salinas, José María
dc.contributor.author	Marchio, Patricia
dc.contributor.author	Iradi Casal, Antonio
dc.contributor.author	Aldasoro, Constanza
dc.contributor.author	Jorda, Adrian
dc.contributor.author	Obrador, Elena
dc.contributor.author	Vallés Martí, Lilián Soraya
dc.date.accessioned	2016-06-27T11:44:51Z
dc.date.available	2016-06-27T11:44:51Z
dc.date.issued	2016
dc.identifier.citation	Aldasoro Celaya, Martín; Guerra-Ojeda, Sol; Aguirre Rueda, Diana; Mauricio, María Dolores; Vila Salinas, José María; Marchio, Patricia; Iradi, Antonio; Aldasoro, Constanza; Jordá, Adrián; Obrador, Elena; Valles, Soraya L. (2016) Effects of ranolazine on astrocytes and neurons in primary culture Plos One 11 3 1 15
dc.identifier.uri	http://hdl.handle.net/10550/54264
dc.description.abstract	Ranolazine (Rn) is an antianginal agent used for the treatment of chronic angina pectoris when angina is not adequately controlled by other drugs. Rn also acts in the central nervous system and it has been proposed for the treatment of pain and epileptic disorders. Under the hypothesis that ranolazine could act as a neuroprotective drug, we studied its effects on astrocytes and neurons in primary culture. We incubated rat astrocytes and neurons in primary cultures for 24 hours with Rn (10−7, 10−6 and 10−5 M). Cell viability and proliferation were measured using trypan blue exclusion assay, MTT conversion assay and LDH release assay. Apoptosis was determined by Caspase 3 activity assay. The effects of Rn on proinflammatory mediators IL-β and TNF-α was determined by ELISA technique, and protein expression levels of Smac/Diablo, PPAR-γ, Mn-SOD and Cu/Zn-SOD by western blot technique. In cultured astrocytes, Rn significantly increased cell viability and proliferation at any concentration tested, and decreased LDH leakage, Smac/Diablo expression and Caspase 3 activity indicating less cell death. Rn also increased anti-inflammatory PPAR-γ protein expression and reduced pro-inflammatory proteins IL-1 β and TNFα levels. Furthermore, antioxidant proteins Cu/Zn-SOD and Mn-SOD significantly increased after Rn addition in cultured astrocytes. Conversely, Rn did not exert any effect on cultured neurons. In conclusion, Rn could act as a neuroprotective drug in the central nervous system by promoting astrocyte viability, preventing necrosis and apoptosis, inhibiting inflammatory phenomena and inducing anti-inflammatory and antioxidant agents.
dc.language.iso	eng
dc.relation.ispartof	Plos One, 2016, vol. 11, num. 3, p. 1-15
dc.subject	Fisiologia humana
dc.title	Effects of ranolazine on astrocytes and neurons in primary culture
dc.type	journal article	es_ES
dc.date.updated	2016-06-27T11:44:51Z
dc.identifier.doi	10.1371/journal.pone.0150619
dc.identifier.idgrec	111058
dc.rights.accessRights	open access	es_ES