NAGIOS: RODERIC FUNCIONANDO

Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation

dc.contributor.author	Klingberg, Franco
dc.contributor.author	Chow, Melissa L.
dc.contributor.author	Koehler, Anne
dc.contributor.author	Boo, Stellar
dc.contributor.author	Buscemi, Lara
dc.contributor.author	Quinn, Thomas M.
dc.contributor.author	Costell Rosselló, Mercedes
dc.contributor.author	Alman, Benjamin A.
dc.contributor.author	Genot, Elisabeth
dc.contributor.author	Hinz, Boris
dc.date.accessioned	2016-07-19T15:59:39Z
dc.date.available	2016-07-19T15:59:39Z
dc.date.issued	2014
dc.identifier.citation	Klingberg, Franco Chow, Melissa L. Koehler, Anne Boo, Stellar Buscemi, Lara Quinn, Thomas M. Costell Rosselló, Mercedes Alman, Benjamin A. Genot, Elisabeth Hinz, Boris 2014 Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation Journal of Cell Biology 207 2 283 297
dc.identifier.uri	http://hdl.handle.net/10550/54633
dc.description.abstract	Integrin-mediated force application induces a conformational change in latent TGF-β1 that leads to the release of the active form of the growth factor from the extracellular matrix (ECM). Mechanical activation of TGF-β1 is currently understood as an acute process that depends on the contractile force of cells. However, we show that ECM remodeling, preceding the activation step, mechanically primes latent TGF-β1 akin to loading a mechanical spring. Cell-based assays and unique strain devices were used to produce a cell-derived ECM of controlled organization and prestrain. Mechanically conditioned ECM served as a substrate to measure the efficacy of TGF-β1 activation after cell contraction or direct force application using magnetic microbeads. The release of active TGF-β1 was always higher from prestrained ECM as compared with unorganized and/or relaxed ECM. The finding that ECM prestrain regulates the bioavailability of TGF-β1 is important to understand the context of diseases that involve excessive ECM remodeling, such as fibrosis or cancer.
dc.language.iso	eng
dc.relation.ispartof	Journal of Cell Biology, 2014, vol. 207, num. 2, p. 283-297
dc.subject	Cèl·lules
dc.subject	Membranes (Biologia)
dc.title	Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation
dc.type	journal article	es_ES
dc.date.updated	2016-07-19T15:59:40Z
dc.identifier.doi	10.1083/jcb.201402006
dc.identifier.idgrec	111999
dc.rights.accessRights	open access	es_ES