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Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques

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Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques

dc.contributor.author	Marín, María José	es
dc.contributor.author	Figuero-Ruiz, Elena	es
dc.contributor.author	González, Itziar	es
dc.contributor.author	O'Connor, Ana	es
dc.contributor.author	Diz Dios, Pedro	es
dc.contributor.author	Álvarez Fernández, Maximiliano	es
dc.contributor.author	Herrera, David	es
dc.contributor.author	Sanz Alonso, Mariano	es
dc.date.accessioned	2016-07-26T11:58:24Z
dc.date.available	2016-07-26T11:58:24Z
dc.date.issued	2016	es
dc.identifier.citation	Marín, María José ; Figuero-Ruiz, Elena ; González, Itziar ; O'Connor, Ana ; Diz Dios, Pedro ; Álvarez Fernández, Maximiliano ; Herrera, David ; Sanz Alonso, Mariano. Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques. En: Medicina oral, patología oral y cirugía bucal. Ed inglesa, 2016, Vol. 21, No. 3: 276-	es
dc.identifier.uri	http://hdl.handle.net/10550/54770
dc.description.abstract	Background: The prevalence and amounts of periodontal pathogens detected in bacteraemia samples after tooth brushing-induced by means of four diagnostic technique, three based on culture and one in a molecular-based technique, have been compared in this study. Material and Methods: Blood samples were collected from thirty-six subjects with different periodontal status (17 were healthy, 10 with gingivitis and 9 with periodontitis) at baseline and 2 minutes after tooth brushing. Each sample was analyzed by three culture-based methods [direct anaerobic culturing (DAC), hemo-culture (BACTEC), and lysis-centrifugation (LC)] and one molecular-based technique [quantitative polymerase chain reaction (qPCR)]. With culture any bacterial isolate was detected and quantified, while with qPCR only Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were detected and quantified. Descriptive analyses, ANOVA and Chi-squared tests, were performed. Results: Neither BACTEC nor qPCR detected any type of bacteria in the blood samples. Only LC (2.7%) and DAC (8.3%) detected bacteraemia, although not in the same patients. Fusobacterium nucleatum was the most frequently detected bacterial species. Conclusions: The disparity in the results when the same samples were analyzed with four different microbiological detection methods highlights the need for a proper validation of the methodology to detect periodontal pathogens in bacteraemia samples, mainly when the presence of periodontal pathogens in blood samples after tooth brushing was very seldom.	en_US
dc.relation		es
dc.subject	Odontología	es
dc.subject	Ciencias de la salud	es
dc.title	Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques	es
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.type.hasVersion	VoR	es_ES