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Biological insertion of computationally designed short transmembrane segments

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Biological insertion of computationally designed short transmembrane segments

dc.contributor.author	Baeza Delgado, Carlos
dc.contributor.author	Heijne, Gunnar von
dc.contributor.author	Martí Renom, Marc A.
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2016-09-06T15:20:33Z
dc.date.available	2016-09-06T15:20:33Z
dc.date.issued	2016
dc.identifier.citation	Baeza Delgado, Carlos Heijne, Gunnar von Marti-Renom, Marc A. Mingarro Muñoz, Ismael 2016 Biological insertion of computationally designed short transmembrane segments Scientific Reports 6 23397 1 9
dc.identifier.uri	http://hdl.handle.net/10550/54924
dc.description.abstract	The great majority of helical membrane proteins are inserted co-translationally into the ER membrane through a continuous ribosome-translocon channel. The efficiency of membrane insertion depends on transmembrane (TM) helix amino acid composition, the helix length and the position of the amino acids within the helix. In this work, we conducted a computational analysis of the composition and location of amino acids in transmembrane helices found in membrane proteins of known structure to obtain an extensive set of designed polypeptide segments with naturally occurring amino acid distributions. Then, using an in vitro translation system in the presence of biological membranes, we experimentally validated our predictions by analyzing its membrane integration capacity. Coupled with known strategies to control membrane protein topology, these findings may pave the way to de novo membrane protein design.
dc.language.iso	eng
dc.relation.ispartof	Scientific Reports, 2016, vol. 6, num. 23397, p. 1-9
dc.subject	Proteïnes de membrana
dc.subject	Membranes (Biologia)
dc.subject	Biofísica
dc.title	Biological insertion of computationally designed short transmembrane segments
dc.type	journal article	es_ES
dc.date.updated	2016-09-06T15:20:33Z
dc.identifier.doi	10.1038/srep23397
dc.identifier.idgrec	110645
dc.rights.accessRights	open access	es_ES