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The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes

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The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes

dc.contributor.author	Peiró, Ana
dc.contributor.author	Martínez Gil, Luis
dc.contributor.author	Tamborero Capilla, Silvia
dc.contributor.author	Pallás Benet, Vicente
dc.contributor.author	Sánchez Navarro, Jesús A.
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2016-09-07T17:45:08Z
dc.date.available	2016-09-07T17:45:08Z
dc.date.issued	2014
dc.identifier.citation	Peiró, Ana Martínez Gil, Luis Tamborero Capilla, Silvia Pallás Benet, Vicente Sánchez Navarro, Jesús A. Mingarro Muñoz, Ismael 2014 The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes Journal of Virology 88 5 3016 3026
dc.identifier.uri	http://hdl.handle.net/10550/54940
dc.description.abstract	Plant positive-strand RNA viruses require association with plant cell endomembranes for viral translation and replication, as well as for intra- and intercellular movement of the viral progeny. The membrane association and RNA binding of the Tobacco mosaic virus (TMV) movement protein (MP) are vital for orchestrating the macromolecular network required for virus movement. A previously proposed topological model suggests that TMV MP is an integral membrane protein with two putative α-helical transmembrane (TM) segments. Here we tested this model using an experimental system that measured the efficiency with which natural polypeptide segments were inserted into the ER membrane under conditions approximating the in vivo situation, as well as in planta. Our results demonstrated that the two hydrophobic regions (HRs) of TMV MP do not span biological membranes. We further found that mutations to alter the hydrophobicity of the first HR modified membrane association and precluded virus movement. We propose a topological model in which the TMV MP HRs intimately associate with the cellular membranes, allowing maximum exposure of the hydrophilic domains of the MP to the cytoplasmic cellular components.
dc.language.iso	eng
dc.relation.ispartof	Journal of Virology, 2014, vol. 88, num. 5, p. 3016-3026
dc.subject	Virus
dc.subject	Microbiologia
dc.title	The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes
dc.type	journal article	es_ES
dc.date.updated	2016-09-07T17:45:08Z
dc.identifier.doi	10.1128/JVI.03648-13
dc.identifier.idgrec	100296
dc.rights.accessRights	open access	es_ES