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Distant downstream sequence determinants can control N-tail translocation during protein insertion into the Endoplasmic Reticulum membrane

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Distant downstream sequence determinants can control N-tail translocation during protein insertion into the Endoplasmic Reticulum membrane

dc.contributor.author	Nilsson, IngMarie
dc.contributor.author	Witt, Susanne
dc.contributor.author	Kiefer, Hans
dc.contributor.author	Mingarro Muñoz, Ismael
dc.contributor.author	Heijne, Gunnar von
dc.date.accessioned	2016-09-08T13:51:19Z
dc.date.available	2016-09-08T13:51:19Z
dc.date.issued	2000
dc.identifier.citation	Nilsson, IngMarie Witt, Susanne Kiefer, Hans Mingarro Muñoz, Ismael Heijne, Gunnar von 2000 Distant downstream sequence determinants can control N-tail translocation during protein insertion into the Endoplasmic Reticulum membrane Journal of Biological Chemistry 275 9 6207 6213
dc.identifier.uri	http://hdl.handle.net/10550/54965
dc.description.abstract	We have studied the membrane insertion of ProW, an Escherichia coli inner membrane protein with seven transmembrane segments and a large periplasmic N-terminal tail, into endoplasmic reticulum (ER)-derived dog pancreas microsomes. Strikingly, significant levels of N-tail translocation is seen only when a minimum of four of the transmembrane segments are present; for constructs with fewer transmembrane segments, the N-tail remains mostly nontranslocated and the majority of the molecules adopt an 'inverted' topology where normally nontranslocated parts are translocated and vice versa. N-tail translocation can also be promoted by shortening of the N-tail and by the addition of positively charged residues immediately downstream of the first trasnmembrane segment. We conclude that as many as four consecutive transmembrane segments may be collectively involved in determining membrane protein topology in the ER and that the effects of downstream sequence determinants may vary depending on the size and charge of the N-tail. We also provide evidence to suggest that the ProW N-tail is translocated across the ER membrane in a C-to-N-terminal direction.
dc.language.iso	eng
dc.relation.ispartof	Journal of Biological Chemistry, 2000, vol. 275, num. 9, p. 6207-6213
dc.subject	Bioquímica
dc.subject	Proteïnes
dc.subject	Membranes (Biologia)
dc.title	Distant downstream sequence determinants can control N-tail translocation during protein insertion into the Endoplasmic Reticulum membrane
dc.type	journal article	es_ES
dc.date.updated	2016-09-08T13:51:20Z
dc.identifier.doi	10.1074/jbc.275.9.6207
dc.identifier.idgrec	003977
dc.rights.accessRights	open access	es_ES