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Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV)

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Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV)

dc.contributor.author	Martínez Gil, Luis
dc.contributor.author	Saurí Peris, Ana
dc.contributor.author	Vilar, Marçal
dc.contributor.author	Pallás Benet, Vicente
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2016-11-29T16:29:37Z
dc.date.available	2016-11-29T16:29:37Z
dc.date.issued	2007
dc.identifier.citation	Martínez Gil, Luis Saurí Peris, Ana Vilar, Marçal Pallás Benet, Vicente Mingarro Muñoz, Ismael 2007 Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV) Virology 367 2 348 357
dc.identifier.uri	http://hdl.handle.net/10550/56205
dc.description.abstract	Cell-to-cell movement of the Melon Necrotic Spot Virus (MNSV) is controlled by two small proteins working in trans, an RNA-binding protein (p7A) and an integral membrane protein (p7B) separated by an amber stop codon. p7B contains a single hydrophobic region. Membrane integration of this region was observed when inserted into model proteins in the presence of microsomal membranes. Furthermore, we explored the topology and targeting mechanisms of full-length p7B. Here we present evidence that p7B integrates in vitro into the ER membrane cotranslationally and with an Nt-cytoplasmic/Ct-luminal orientation. The observed topology was monitored in vivo by fusing GFP to the Ct of p7B, enabling the overexpression in Escherichia coli cultures. Finally, the topology of a putative p14 movement protein was established by replacing the amber stop codon located between p7A and p7B.
dc.language.iso	eng
dc.relation.ispartof	Virology, 2007, vol. 367, num. 2, p. 348-357
dc.subject	Proteïnes de membrana
dc.subject	Virus
dc.title	Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV)
dc.type	journal article	es_ES
dc.date.updated	2016-11-29T16:29:37Z
dc.identifier.doi	10.1016/j.virol.2007.06.006
dc.identifier.idgrec	039203
dc.rights.accessRights	open access	es_ES