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Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECT in Guinea Pig: Role of Bitter Taste Agonists

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Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECT in Guinea Pig: Role of Bitter Taste Agonists

dc.contributor.author	Ortiz Belda, José Luis
dc.contributor.author	Ortiz, Amparo
dc.contributor.author	Milara Payá, Javier
dc.contributor.author	Armengot Carceller, Miguel
dc.contributor.author	Sanz García, Celia
dc.contributor.author	Compañ Quilis, Desamparados
dc.contributor.author	Morcillo Sánchez, Esteban
dc.contributor.author	Cortijo Domingo, Julio
dc.date.accessioned	2017-01-30T07:49:42Z
dc.date.available	2017-01-30T07:49:42Z
dc.date.issued	2016
dc.identifier.citation	Ortiz Belda, José Luis Ortiz, Amparo Milara Payá, Javier Armengot Carceller, Miguel Sanz García, Celia Compañ Quilis, Desamparados Morcillo Sánchez, Esteban Jesús Cortijo Domingo, Julio 2016 Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECT in Guinea Pig: Role of Bitter Taste Agonists Plos One 11 10
dc.identifier.uri	http://hdl.handle.net/10550/56938
dc.description.abstract	Different image techniques have been used to analyze mucociliary clearance (MCC) in humans, but current small animal MCC analysis using in vivo imaging has not been well defined. Bitter taste receptor (T2R) agonists increase ciliary beat frequency (CBF) and cause bronchodilation but their effects in vivo are not well understood. This work analyzes in vivo nasal and bronchial MCC in guinea pig animals using three dimension (3D) micro-CT-SPECT images and evaluates the effect of T2R agonists. Intranasal macroaggreggates of albumin-Technetium 99 metastable (MAA-Tc99m) and lung nebulized Tc99m albumin nanocolloids were used to analyze the effect of T2R agonists on nasal and bronchial MCC respectively, using 3D micro-CT-SPECT in guinea pig. MAA-Tc99m showed a nasal mucociliary transport rate of 0.36 mm/min that was increased in presence of T2R agonist to 0.66 mm/min. Tc99m albumin nanocolloids were homogeneously distributed in the lung of guinea pig and cleared with time-dependence through the bronchi and trachea of guinea pig. T2R agonist increased bronchial MCC of Tc99m albumin nanocolloids. T2R agonists increased CBF in human nasal ciliated cells in vitro and induced bronchodilation in human bronchi ex vivo. In summary, T2R agonists increase MCC in vivo as assessed by 3D micro-CT-SPECT analysis.
dc.language.iso	eng
dc.relation.ispartof	Plos One, 2016, vol. 11, num. 10
dc.title	Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECT in Guinea Pig: Role of Bitter Taste Agonists
dc.type	journal article	es_ES
dc.date.updated	2017-01-30T07:49:42Z
dc.identifier.doi	10.1371/journal.pone.0164399
dc.identifier.idgrec	115671
dc.rights.accessRights	open access	es_ES