Background: Evaluate the influence of different hybridization bonding techniques of a self-adhesive resin cement. Material and Methods: 30 human health molars were divided into six groups (n=10). The specimens received three longitudinal sections, allowing insertion of central cuts in PVC matrices. Each group received a different dentin pretreatment according to the manufacturerâ s recommendations, except the control group (G1), as follows. G2 - a 3-step total-etch adhesive system (Optibondâ ¢ FL, Kerr); G3 - a 3-step total-etch adhesive system (Adperâ ¢ Scotchbondâ ¢ Multi-Purpose, 3M ESPE); G4 - a 2-step total-etch adhesive system (Adperâ ¢ Single Bond 2, 3M ESPE); G5 - a single-step self-etching system (Bond Force, Tokuyama); and G6 - universal bonding system (Single Bond Universal, 3M ESPE). Then, cylinders made of self-adhesive resin cement with polypropylene matrix was cemented in all groups (RelyX U200, 3M ESPE). Bond strength was assessed by submitting the specimens to micro-shear test and was characterized according to the fracture pattern observed through optical microscopy. Results: The results were submitted to the Kruskal-Wallis test, which indicated a statistically significant difference between the groups (p=0.04), and Tukeyâ s multiple comparisons, which indicated a statistically significant difference between G1 and G3 (p<0.05). The microscopic analysis revealed a high prevalence of adhesive failures, followed by mixed fractures, and cohesive failures in the dentin. Conclusions: The use of a previous dentin hybridization protocol is able to increase adhesive bonding resistance of self-adhesive resin cement, especially when used Adperâ ¢ Scotchbondâ ¢ Multi-Purpose system.