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Effects of asymmetric dimethylarginine on renal arteries in portal hypertension and cirrhosis

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Effects of asymmetric dimethylarginine on renal arteries in portal hypertension and cirrhosis

dc.contributor.author	Segarra Irles, Gloria Vicenta
dc.contributor.author	Cortina Gil, Belén
dc.contributor.author	Mauricio Aviñó, María Dolores
dc.contributor.author	Novella del Campo, Susana
dc.contributor.author	Lluch García, Paloma
dc.contributor.author	Navarrete-Navarro, Javier
dc.contributor.author	Noguera Salvá, Inmaculada
dc.contributor.author	Medina Bessó, Pascual
dc.date.accessioned	2017-05-08T09:42:39Z
dc.date.available	2017-05-08T09:42:39Z
dc.date.issued	2016
dc.identifier.citation	Segarra Irles, Gloria Vicenta Cortina Gil, Belén Mauricio Aviñó, Maria Dolores Novella del Campo, Susana Lluch García, Paloma Navarrete-Navarro, Javier Noguera Salvá, Inmaculada Medina Bessó, Pascual 2016 Effects of asymmetric dimethylarginine on renal arteries in portal hypertension and cirrhosis World Journal of Gastroenterology 22 48 10545 10556
dc.identifier.uri	http://hdl.handle.net/10550/58386
dc.description.abstract	AIM. To evaluate the effects of asymmetric dimethylarginine (ADMA) in renal arteries from portal hypertensive and cirrhotic rats. METHODS. Rat renal arteries from Sham (n = 15), pre-hepatic portal hypertension (PPVL; n = 15) and bile duct ligation and excision-induced cirrhosis (BDL; n = 15) were precontracted with norepinephrine, and additional contractions were induced with ADMA (10-6-10-3 mol/L), an endogenous inhibitor of nitric oxide (NO) synthase. Concentration-response curves to acetylcholine (1 × 10-9-3 × 10-6 mol/L) were determined in precontracted renal artery segments with norepinephrine in the absence and in the presence of ADMA. Kidneys were collected to determine the protein expression and activity of dimethylarginine dimethylaminohydrolase (DDAH), an enzyme that catabolizes ADMA. RESULTS. In renal arteries precontracted with norepinephrine, ADMA caused endothelium-dependent contractions. The pD2 values to ADMA were similar in the Sham and PPVL groups (4.20 ± 0.08 and 4.11 ± 0.09, P > 0.05, respectively), but were lower than those of the BDL group (4.79 ± 0.16, P < 0.05). Acetylcholine-induced endothelium-dependent relaxation that did not differ, in terms of pD2 and maximal relaxation, among the 3 groups studied. Treatment with ADMA (3 × 10-4 mol/L) inhibited acetylcholine-induced relaxation in the 3 groups, but the inhibition was higher (P < 0.05) in the BDL group compared with that for the Sham and PPVL groups. The mRNA and protein expression of DDAH-1 were similar in kidneys from the three groups. Conversely, DDAH-2 expression was increased (P < 0.05) in PPVL and further enhanced (P < 0.05) in the BDL group. However, renal DDAH activity was significantly decreased in the BDL group. CONCLUSION. Cirrhosis increased the inhibitory effect of ADMA on basal- and induced-release of NO in renal arteries, and decreased DDAH activity in the kidney.
dc.language.iso	eng
dc.relation.ispartof	World Journal of Gastroenterology, 2016, vol. 22, num. 48, p. 10545-10556
dc.subject	Cirrosi hepàtica
dc.subject	Pressió sanguínia
dc.title	Effects of asymmetric dimethylarginine on renal arteries in portal hypertension and cirrhosis
dc.type	journal article	es_ES
dc.date.updated	2017-05-08T09:42:40Z
dc.identifier.doi	10.3748/wjg.v22.i48.10545
dc.identifier.idgrec	116222
dc.rights.accessRights	open access	es_ES