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Phylogenetic analysis of an epidemic outbreak of acute hepatitis C in HIV-infected patients by ultra-deep pyrosequencing

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Phylogenetic analysis of an epidemic outbreak of acute hepatitis C in HIV-infected patients by ultra-deep pyrosequencing

dc.contributor.author	Caro-Pérez, Noelia
dc.contributor.author	Martínez-Rebollar, María
dc.contributor.author	Gregori, Josep
dc.contributor.author	Quer, J.
dc.contributor.author	González, Patricia
dc.contributor.author	Gambato, Martina
dc.contributor.author	García-González, Neris
dc.contributor.author	González Candelas, Fernando
dc.contributor.author	Costa, Josep
dc.contributor.author	Esteban, Juan Ignacio
dc.contributor.author	Mallolas, Josep
dc.contributor.author	Forns, Xavier
dc.contributor.author	Laguno, Montse
dc.contributor.author	Pérez-del-Pulgar, Sofía
dc.date.accessioned	2017-06-01T14:01:54Z
dc.date.available	2017-06-01T14:01:54Z
dc.date.issued	2017
dc.identifier.citation	Caro-Pérez, Noelia Martínez-Rebollar, María Gregori, Josep Quer, J. González, Patricia Gambato, Martina García-González, Neris González Candelas, Fernando Costa, Josep Esteban, Juan Ignacio Mallolas, Josep Forns, Xavier Laguno, Montse Pérez-del-Pulgar, Sofía 2017 Phylogenetic analysis of an epidemic outbreak of acute hepatitis C in HIV-infected patients by ultra-deep pyrosequencing Journal of Clinical Virology 92 42 47
dc.identifier.uri	http://hdl.handle.net/10550/58805
dc.description.abstract	Background: The incidence of acute hepatitis C (AHC) among HIV-infected men who have sex with men (MSM) has increased significantly in the last 10 years. Several studies point to a social and sexual network of HIV-positive MSM that extends internationally. Objectives: The aim of our study was to investigate the dynamics of HCV transmission in an outbreak of AHC in HIV-infected MSM in Barcelona by ultra-deep pyrosequencing. Study design: Between 2008 and 2013, 113 cases of AHC in HIV-infected MSM were diagnosed in the Infectious Diseases Unit, Hospital Clínic, Barcelona. Massive sequencing was performed using the Roche 454 GS Junior platform. To define possible transmission networks, maximum likelihood phylogenetic trees were constructed, and levels of genetic diversity within and among patients were compared. Results: Among the 70 cases analyzed, we have identified 16 potential clusters of transmission: 8 for genotype 1a (23 cases involved), 1 for genotype 1b (3 cases) and 7 for genotype 4d (27 cases). Although the initial phylogenetic reconstruction suggested a local transmission cluster of HCV gt4d, our approach based on low genetic differentiation did not corroborate it. Indeed, gt4d strains formed 4 independent groups related to patients from other countries. Conclusions: Frequent clustering of HIV-positive MSM shows that HCV infection has spread through a local network in Barcelona. This outbreak is related to a large international HCV transmission network among MSM. Public health efforts are needed to reduce HCV transmission among this high-risk group.
dc.language.iso	eng
dc.relation.ispartof	Journal of Clinical Virology, 2017, vol. 92, p. 42-47
dc.subject	Virus
dc.subject	Biotecnologia
dc.subject	Malalties de transmissió sexual
dc.title	Phylogenetic analysis of an epidemic outbreak of acute hepatitis C in HIV-infected patients by ultra-deep pyrosequencing
dc.type	journal article	es_ES
dc.date.updated	2017-06-01T14:01:54Z
dc.identifier.doi	10.1016/j.jcv.2017.05.008
dc.identifier.idgrec	117890
dc.rights.accessRights	open access	es_ES