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Growth kinetics and characterization of human dental pulp stem cells: comparison between third molar and first premolar teeth

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Growth kinetics and characterization of human dental pulp stem cells: comparison between third molar and first premolar teeth

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dc.contributor.author Mehrabani, Davood es
dc.contributor.author Mahdiyar, Parisa es
dc.contributor.author Torabi, Kianoosh es
dc.contributor.author Robati, Reza es
dc.contributor.author Zare, Shahrokh es
dc.contributor.author Dianatpour, Mehdi es
dc.contributor.author Tamadon, Amin es
dc.date.accessioned 2017-07-18T12:20:54Z
dc.date.available 2017-07-18T12:20:54Z
dc.date.issued 2017 es
dc.identifier.citation Mehrabani, Davood ; Mahdiyar, Parisa ; Torabi, Kianoosh ; Robati, Reza ; Zare, Shahrokh ; Dianatpour, Mehdi ; Tamadon, Amin. Growth kinetics and characterization of human dental pulp stem cells: comparison between third molar and first premolar teeth. En: Journal of Clinical and Experimental Dentistry. 2017. Vol. 9, no. 2: 172 es
dc.identifier.uri http://hdl.handle.net/10550/59611
dc.description.abstract Dental pulp stem cells (DPSCs) play an important role in tissue regeneration. This study compares the growth kinetics and characterization of third molar and first premolar human DPSCs. Dental pulp tissues were isolated from human first premolar and third molar teeth and were digested by treating them with collagenase type I. Single-cell suspensions from each dental pulp were seeded in T25 culture flasks and the media were replaced every 3 days until 70% confluence. The cells were enumerated to determine the population doubling time (PDT). Cells were characterized using flow cytometry, RT-PCR and osteogenic medium for differentiation of DPSCs. Karyotyping assay was also performed till passage 7th. The DPSCs had spindle-shaped morphology. There was an increase in PDT in third molar DPSCs when compared to first premolar teeth. Positive expression of CD44, CD73, and CD90 and negative expression of CD34 and CD45 were illustrated. A normal karyotype was visible for all seven passages. The Alizarin red staining was positive for osteogenic induction of DPSCs. When DPSCs are needed, third molar teeth can be a good and convenient candidate for cell transplantation, yielding high number of cells with mesenchymal characteristics. They can be a source for further investigations in vitro and work on tissue engineering protocols. en_US
dc.subject Odontología es
dc.subject Ciencias de la salud es
dc.title Growth kinetics and characterization of human dental pulp stem cells: comparison between third molar and first premolar teeth es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.type.hasVersion VoR es_ES

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