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Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts

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Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts

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dc.contributor.author Samiei, Mohammad es
dc.contributor.author Ghasemi, Negin es
dc.contributor.author Aghazadeh, Marzieh es
dc.contributor.author Divband, Baharak es
dc.contributor.author Akbarzadeh, Farzaneh es
dc.date.accessioned 2017-07-18T12:20:55Z
dc.date.available 2017-07-18T12:20:55Z
dc.date.issued 2017 es
dc.identifier.citation Samiei, Mohammad ; Ghasemi, Negin ; Aghazadeh, Marzieh ; Divband, Baharak ; Akbarzadeh, Farzaneh. Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts. En: Journal of Clinical and Experimental Dentistry. 2017. Vol. 9, no. 2: 182 es
dc.identifier.uri http://hdl.handle.net/10550/59613
dc.description.abstract The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA’s ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at different storage times after mixing on human gingival fibroblasts (HGFs). HGFs were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco’s Modified Eagle medium, supplemented with 10% fetal calf serum, penicillin and streptomycin. The cells were exposed to WMTA (groups 1 and 2) and WMTA+TiO2 (groups 3 and 4). The fifth and sixth groups served as controls. Each group contained 15 wells. After 24h (groups 1, 3 and 5) and 48 h (groups 2, 4 and 6) of exposure, HGF viability was determined by Mosmann’s tetrazolium toxicity (MTT) assay. Statistical analysis of the data was performed by using one-way analysis of variance and Tukey post hoc test, with significance of p < 0.05. With both materials, the viability of HGFs significantly decrased with increasing the incubation time from 24h to 48 h (P<0.05). There was no significant difference between the materials regarding HGF viability (P>0.05). Under the limitations of the present study, incorporation of TiO2 nanoparticles into MTA at 1 wt% had no negative effect on its biocompatibility. en_US
dc.subject Odontología es
dc.subject Ciencias de la salud es
dc.title Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.type.hasVersion VoR es_ES

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