Bleached dental enamel can be more susceptible to staining than the enamel that has never been bleached, especially right after tooth bleaching. The aim of this study is to evaluate the effect of surface treatments and waiting time prior to contact with dye on bleached enamel staining susceptibility. One hundred teeth were bleached with 35% hydrogen peroxide (Whiteness HP, FGM) and randomly assigned to G1 artificial saliva, G2 2% sodium fluoride (Flugel, Nova DFL), G3 casein phosphopeptide-amorphous calcium phosphate fluoride paste (CPP-ACPF, MI Paste Plus, GC America), G4 rinse for color maintenance after bleaching (Keep White Rinse, DMC) and G5 polishing with aluminum oxide-impregnated disks (Super Buff Disk, Shofu). Fifty specimens were immersed in red wine for 15 minutes, immediately after treatment, and the others one hour after. Color difference (?E) was evaluated with a spectrophotometer (Vita EasyShade). Surface treatments and waiting time effects were analyzed with Kruskal-Wallis and Mann Whitney tests (p<0.05). Surface treatments (p>0.05) and waiting time (p>0.05) were not significant to decrease bleached enamel susceptibility to red wine staining. Surface treatments were similar to artificial saliva for bleached enamel susceptibility to red wine staining. Immediate or one-hour-postponed contact with red wine did not affect bleached enamel color.