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IL-4 induces the formation of multinucleated giant cells and expression of ?5 integrin in central giant cell lesion

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IL-4 induces the formation of multinucleated giant cells and expression of ?5 integrin in central giant cell lesion

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dc.contributor.author Aghbali, Amirala es
dc.contributor.author Rafieyan, Sona es
dc.contributor.author Mohamed Khosroshahi, Leila es
dc.contributor.author Baradaran, Behzad es
dc.contributor.author Shanehbandi, Dariush es
dc.contributor.author Kouhsoltani, Maryam es
dc.date.accessioned 2017-07-21T11:50:14Z
dc.date.available 2017-07-21T11:50:14Z
dc.date.issued 2017 es
dc.identifier.citation Aghbali, Amirala ; Rafieyan, Sona ; Mohamed Khosroshahi, Leila ; Baradaran, Behzad ; Shanehbandi, Dariush ; Kouhsoltani, Maryam. IL-4 induces the formation of multinucleated giant cells and expression of ?5 integrin in central giant cell lesion. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 22 1 2017: 4- es
dc.identifier.uri http://hdl.handle.net/10550/59792
dc.description.abstract It is now well established that IL-4 has a central role in the development of monocytes to multinucleated giant cells (MGCs) by inducing the expression of integrins on the surface of monocytes. The aim of this study was to investigate the potential role of IL-4 in induction of ?5 integrin expression in the peripheral blood samples of patients with giant cell granuloma. Monocytes were isolated from peripheral blood samples of patients with central giant cell granuloma (CGCG) and healthy controls using human Monocyte Isolation Kit II. Isolated monocytes were then cultured in the absence or presence of IL-4 (10 and 20 ng/mL), and following RNA extraction and cDNA synthesis, Real-time PCR was performed to determine the level of ?5 integrin expression. The formation of CGCGs and morphological analyses were done under light microscopy. For confirmation of CGCGs, immunocytochemistry technique was also carried out by anti-RANK (receptor-activator of NF-?B ligand) antibody. In both patient and control groups, ?5 levels were significantly enhanced by increasing the IL-4 dose from 10 to 20 ng/mL. In addition, these differences were significant between patient and control groups without IL-4 treatment. On the other hand, the number of cells which expressed RANK and therefore the number of giant cells were significantly higher in the patient group in comparison to controls, as assessed by immunohistochemistry evaluations. In this study, we showed an elevation in the expression levels of ?5 integrin when stimulated by IL-4. It is strongly indicated that this integrin acts as an important mediator during macrophage to macrophage fusion and development of giant cells. es
dc.title IL-4 induces the formation of multinucleated giant cells and expression of ?5 integrin in central giant cell lesion es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.20935 es
dc.type.hasVersion VoR es_ES

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