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An in vitro study showing the three-dimensional microenvironment influence over the behavior of head and neck squamous cell carcinoma

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An in vitro study showing the three-dimensional microenvironment influence over the behavior of head and neck squamous cell carcinoma

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dc.contributor.author Salgueiredo-Giudice, Fernanda es
dc.contributor.author Corrêa-Abrahão, Aline es
dc.contributor.author Sperandio, Felipe-Fornias es
dc.contributor.author da-Costa-Dal-Vechio, Aluana Maria es
dc.contributor.author Júnior, Décio-dos-Santos-Pinto es
dc.date.accessioned 2017-07-25T10:17:37Z
dc.date.available 2017-07-25T10:17:37Z
dc.date.issued 2012 es
dc.identifier.citation Salgueiredo-Giudice, Fernanda ; Corrêa-Abrahão, Aline ; Sperandio, Felipe-Fornias ; da-Costa-Dal-Vechio, Aluana Maria ; Júnior, Décio-dos-Santos-Pinto. An in vitro study showing the three-dimensional microenvironment influence over the behavior of head and neck squamous cell carcinoma. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 17 3 2012: 16- es
dc.identifier.uri http://hdl.handle.net/10550/59949
dc.description.abstract Objectives: The Head and Neck Squamous Cell Carcinoma (HNSCC) ranks sixth worldwide. The mechanisms of growth, invasion and metastasis of this pathology are extensively studied and generally related to specific variations in signaling pathways like the PI3K-Akt; however most of these competent studies have been performed bidimensionally, which may hide important questions. This study sought to analyze the influence of the microenvironment upon the behavior of HNSCC. Study Design: The status of pAkt, NF-?B and Cyclin D1 proteins was accessed through immunofluorescence and western blot methods in HNSCC cell lines originating from tongue, pharynx and metastatic lymph node when submitted to a three-dimensional culture model utilizing a matrix system. A bidimensional culture model (monolayer) was used as control. Results: The HNSCC cell lines cultured three-dimensionally exhibited a growth pattern characterized by small isolated islands, different from the control group. When the three-dimensional model was applied, two of the studied cell lines showed the same expression pattern as the bidimensional model regarding nuclear or cytoplasmatic localization, as well as reduction of all protein levels; however, the cell line originated from tongue, which specially has the epidermal growth factor receptor constitutively activated, demonstrated nuclear translocation of pAkt and also an increase in the levels of Cyclin D1. Conclusions: The results suggest the influence of the microenvironment upon the behavior of HNSCC cells due to the changed expression of proteins related to tumor growth and cellular invasion. Furthermore, intrinsically genetic conditions also played important roles over the cells, despite the culture model employed. es
dc.title An in vitro study showing the three-dimensional microenvironment influence over the behavior of head and neck squamous cell carcinoma es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.17538 es
dc.type.hasVersion VoR es_ES

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