Objectives: To develop and to evaluate a questionnaire of self-perception and self-care habits on oral health on a first level population. Methods: A descriptive observational studas perfored to validate a questionnaire on oral health self-perception (UISESS-B). After non-probabilistic sampling,94 users,30-59 years of age,with either diabetes mellitus 2,hypertension or obesity were included. Duration of disease was lesser than 10 years. Pearson's r, Cornbach's ?, factorial analysis,chi-square and Snedecor's F tests were employed. Results:A Pearson's r of 0.7and Cronbach's ? of 0.82was observed on the pre-post values of the global questionnaire. In the factorial analysis, the variance explained more than 60% for a first factor. Apunctuation of very high risk for the three groups with the scale UISESSS-B that coincides with the index CPO-D and the index UISESS-F was observed. Conclusions: The UISESS-B scale shows significant validity and reliability, suggestingits use as a sensitive ins-trument for the measurement of oral health in people with chronic illnesses such as diabetes,hypertension and obesity. © Medicina Oral S. L.