Objective: The objective of this study was to assess the prevalence and characteristics of dens invaginatus in anterior teeth and to classify the type of dens invaginatus in a sample of Turkish Anatolian population. Study design: A retrospective study was performed using full-mouth periapical and panoramic radiographs of 1012 patients. Maxillary and mandibular anterior teeth were evaluated for the presence and characteristics of dens invaginatus. Statistical evaluation of the presence of dens invaginatus related to gender was performed by the Pearson chi-squared test. Results: Dens invaginatus was observed in 13 out of 1012 subjects and in only maxillary lateral incisors. There were no periapical lesions in teeth with types I and II, whereas both of the subjects with type III had apical periodontitis at the time of referral. Males and females were almost equally affected by dens invaginatus (P= 0.98). Conclusion: The anomaly was detected in only maxillary lateral incisors with no gender difference and the most commonly observed type of dens invaginatus was type I (81.25%).