Objectives: To evaluate the analgesic effect of laser therapy on healthy tissue of mice. Study design: Forty-five animals were divided in three groups of 15: A ? infrared laser irradiation (830 nm, Kondortech®, São Carlos, SP, Brazil); B ? red laser irradiation (660 nm, Kondortech®, São Carlos, SP, Brazil); C ? sham irradiation with laser unit off. After laser application, the mice remained immobilized for the injection of 30 µl of 2% formalin in the plantar pad of the irradiated hind paw. The time that the mouse kept the hind paw lifted was measured at 5 min intervals for 30 minutes. Results: Results showed statistically significant differences comparing the control group with the infrared laser group at 5, 20, 25 and 30 accumulated minutes, and with the red laser group at all time points. The analysis of partial times, at each 5 minutes, showed statistically significant differences between the control and the laser groups up to 20 minutes. Conclusions: Laser therapy had an analgesic effect and red laser had the best results.