Background. Bonding agents could be used as fissure sealants. This study compares the retention three fissure sealants (Delton®, Delton Plus® and Concise®) and a filled dentin bonding system (Optibond Solo®). Methods. Fifty-six children aged 7-8 years received fissure sealants either in the four permanent first molars, in the four deciduous second molars, or in all eight of these teeth. Every child received a different sealing material in each quadrant on a random basis. Clinical evaluation at 12 months was performed by a single blind examiner, and the retention was classified as either a success (total retention) or a failure (partial retention or not present). Results: There were no statistically significant differences among the four materials in permanent maxillary molars or deciduous molars. In permanent mandibular molars, Optibond Solo® showed a lower percentage of retention (40.9%), significantly different (p=0.002) to that of Delton® (89.5%), Delton Plus® (87.5%) and Concise® (76.5%). Conclusion: One bottle dentin bonding system used as a sealant does not improve the retention of conventional fissure sealants. Clinical Implication: Because of the scarcity of studies on the use of dental adhesives as sealants, further studies are warranted for the final support of that conclusion.