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dc.contributor.advisor | Valero Aleixandre, María Adela | |
dc.contributor.advisor | Bargues Castelló, María Dolores | |
dc.contributor.advisor | Mas-Coma, S. | |
dc.contributor.author | Calderón Romero, Leticia | |
dc.contributor.other | Departament de Farmàcia i Tecnologia Farmacèutica | es_ES |
dc.date.accessioned | 2017-11-29T13:26:36Z | |
dc.date.available | 2018-11-30T05:45:05Z | |
dc.date.issued | 2017 | es_ES |
dc.date.submitted | 27-11-2017 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10550/63399 | |
dc.description.abstract | La fascioliasis es una importante enfermedad causada por dos especies de trematodos, Fasciola hepatica y Fasciola gigantica, que incluye un amplio espectro de especies hospedadoras definitivas tanto domésticas como silvestres. En el presente trabajo, como primer paso, se realizó una revisión bibliográfica exhaustiva de todos los casos humanos de fascioliasis en México, reportados tanto en la literatura mexicana, como casos de pacientes mexicanos diagnosticados en Canadá y USA. El estudio fenético y genético se realizó con adultos del parásito, obtenidos de bovinos del matadero de la Ciudad de Toluca, Estado de México, México, zona de endemia humana. Para el estudio fenético comparativo se usaron parásitos adultos de especies standard provenientes de Bóvidos de los siguientes lugares geográficos: a) F. gigantica: Bobo Dioulasso, Burkina Faso (África); F. hepatica: Córcega, Francia (Europa); Valencia, España (Europa): Altiplano Norte Boliviano (América): Se realizó un análisis morfométrico exhaustivo usando un Sistema de Análisis de Imágenes Digitales (CIAS). El análisis genético se efectuó utilizando marcadores genéticos ribosomales (ITS-1, 5.8S, ITS-2). Los resultados de este estudio demuestran la única presencia de F. hepatica, en todas las poblaciones estudiadas de México, no detectándose la presencia de F. gigantica ni de formas intermedias. | es_ES |
dc.description.abstract | Fascioliasis is a plantborne and zoonotic parasitic disease caused by fasciolid liver flukes. Fasciola hepatica is the only fasciolid species described in the Americas, mainly transmitted by lymnaeid snail vectors of the Galba/Fossaria group. Human fascioliasis endemic areas are mainly located in high altitude areas of South America but also North America. Given the necessity to characterize the F. hepatica populations involved, the phenotypic features of fasciolid adults infecting cattle present in fascioliasis endemic areas such as in State of Mexico (Mexico) (valley transmission pattern) were analysed and compared with material from the Northern Bolivian Altiplano (altiplanic transmission pattern). In Mexico, parasites were obtained from the Toluca Valley, located in the central area of the State of Mexico, at a mean altitude of 2,600 m. Numerous permanent water bodies are situated in the area, continuously receiving thaw water from Nevado de Toluca o Xinantécatl volcano (4,200 m altitude) through community supply channels, an epidemiological scenario similar to that previously described in the hyperendemic areas of Andean countries. A computer image analysis system (CIAS) was applied on the basis of standardized measurements. The aforementioned F. hepatica highland populations from Mexico and Bolivia were compared to standard lowland natural populations of European origin (Spain and France) and also to a population of F. gigantica of African origin (Burkina Faso). Liver-fluke size was studied by principal component analysis (PCA). Thus, for the first time, a comparative phenotypic study of the adults of F. hepatica from cattle in Mexico has been carried out. Results are analyzed in terms of permanent/seasonal transmission characteristics. The present work validates the utility of the phenotypic characterization of fasciolid adults in multidisciplinary studies. | en_US |
dc.format.extent | 172 p. | es_ES |
dc.language.iso | es | es_ES |
dc.subject | fascioliasis | es_ES |
dc.subject | humanos | es_ES |
dc.subject | morfometría | es_ES |
dc.subject | bovinos | es_ES |
dc.subject | México | es_ES |
dc.subject | España | es_ES |
dc.subject | Fenotipaje | es_ES |
dc.subject | Genotipaje | es_ES |
dc.title | Caracterización Fenética y Genética de Individuos del Género Fasciola Linnaeus, 1758 (Trematoda: Fasciolidae) de México | es_ES |
dc.type | doctoral thesis | es_ES |
dc.subject.unesco | fascioliasis | es_ES |
dc.subject.unesco | parasitosis | es_ES |
dc.embargo.terms | 1 year | es_ES |