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Estudio experimental comparado de marcadores bioquímicos en la infección por Fasciola hepatica y F. gigantica

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Estudio experimental comparado de marcadores bioquímicos en la infección por Fasciola hepatica y F. gigantica

dc.contributor.advisor	Valero Aleixandre, María Adela
dc.contributor.advisor	Mas-Coma, S.
dc.contributor.author	Quesada Sánchez, Carla
dc.contributor.other	Departament de Biologia Cel.lular i Parasitologia	es_ES
dc.date.accessioned	2017-11-30T13:23:45Z
dc.date.available	2017-12-01T05:45:06Z
dc.date.issued	2017	es_ES
dc.date.submitted	29-11-2017	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/63407
dc.description.abstract	Fascioliasis is caused by the genetically and phenotypically very close Fasciola hepatica and F. gigantica. The latter, always considered secondary in human infection, appears nowadays increasingly involved in human endemic areas of Africa and Asia. Unfortunately, little is known about the pathogenicity of this liver fluke species, mainly due to difficulties assessing the moment of a patient's infection in the anamnesis and in the differential diagnosis with F. hepatica. This is the first experimental study comparing F. hepatica and F. gigantica in a long-term study of up to 24 weeks with genotypically and phenotypically standardised fluke strains in the same animal model host, the Guirra sheep breed susceptible to both species. Serum biochemical parameters of liver damage (aspartate aminotransferase AST, alanine aminotransferase ALT, γ-glutamyl transferase GGT, total bilirubin, alkaline phosphatase AP), serum electrolytes (calcium, chloride, phosphore), protein metabolism (creatinine, blood urea nitrogen), plasma proteins (albumin, total proteins), carbohydrate metabolism (glucose, amylase), hepatic lipid metabolism (total cholesterol, triglycerides) and inflammation (C-reactive protein), were analysed on a biweekly basis as morbidity indicators. Serum anti-Fasciola IgG, coproantigen and egg shedding were simultaneously followed up. DNA sequencing of rDNA ITS-2 and ITS-1 and mtDNA cox1 and nad1, as well as the morphometric study by CIAS, showed the two fasciolid strains used to fit respective standard species characteristics. Results demonstrated that F. gigantica is more pathogenic than F. hepatica, mostly due to its bigger size. Fasciola gigantica shows a delayed development of 1-2 weeks regarding both biliary phase and beginning of egg laying, with the respective consequences on the biochemical marker profile modifications in both the acute and chronic periods. A review on the physiopathogenicity by F. gigantica compared to that by F. hepatica is made. A biochemical marker baseline for human infection by F. gigantica is proposed to help physicians and health officers in F. gigantica human endemic areas.	en_US
dc.description.abstract	Fascioliasis is caused by the genetically and phenotypically very close Fasciola hepatica and F. gigantica. The latter, always considered secondary in human infection, appears nowadays increasingly involved in human endemic areas of Africa and Asia. Unfortunately, little is known about the pathogenicity of this liver fluke species, mainly due to difficulties assessing the moment of a patient's infection in the anamnesis and in the differential diagnosis with F. hepatica. This is the first experimental study comparing F. hepatica and F. gigantica in a long-term study of up to 24 weeks with genotypically and phenotypically standardised fluke strains in the same animal model host, the Guirra sheep breed susceptible to both species. Serum biochemical parameters of liver damage (aspartate aminotransferase AST, alanine aminotransferase ALT, γ-glutamyl transferase GGT, total bilirubin, alkaline phosphatase AP), serum electrolytes (calcium, chloride, phosphore), protein metabolism (creatinine, blood urea nitrogen), plasma proteins (albumin, total proteins), carbohydrate metabolism (glucose, amylase), hepatic lipid metabolism (total cholesterol, triglycerides) and inflammation (C-reactive protein), were analysed on a biweekly basis as morbidity indicators. Serum anti-Fasciola IgG, coproantigen and egg shedding were simultaneously followed up. DNA sequencing of rDNA ITS-2 and ITS-1 and mtDNA cox1 and nad1, as well as the morphometric study by CIAS, showed the two fasciolid strains used to fit respective standard species characteristics. Results demonstrated that F. gigantica is more pathogenic than F. hepatica, mostly due to its bigger size. Fasciola gigantica shows a delayed development of 1-2 weeks regarding both biliary phase and beginning of egg laying, with the respective consequences on the biochemical marker profile modifications in both the acute and chronic periods. A review on the physiopathogenicity by F. gigantica compared to that by F. hepatica is made. A biochemical marker baseline for human infection by F. gigantica is proposed to help physicians and health officers in F. gigantica human endemic areas.	es_ES
dc.format.extent	179 p.	es_ES
dc.language.iso	es	es_ES
dc.subject	fasciola hepatica	es_ES
dc.subject	fasciola gigantica	es_ES
dc.subject	physiopathogenecity	es_ES
dc.subject	morbidity indicators	es_ES
dc.subject	biochemical parameters	es_ES
dc.subject	IgG	es_ES
dc.subject	coproantigen	es_ES
dc.subject	egg shedding	es_ES
dc.subject	sheep experimental infection	es_ES
dc.subject	acute and chronic phases	es_ES
dc.subject	human disease extrapolation	es_ES
dc.title	Estudio experimental comparado de marcadores bioquímicos en la infección por Fasciola hepatica y F. gigantica	es_ES
dc.type	doctoral thesis	es_ES
dc.embargo.terms	0 days	es_ES