NAGIOS: RODERIC FUNCIONANDO

The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation

dc.contributor.author	Gómez-Herreros, Fernando
dc.contributor.author	Margaritis, Thanasis
dc.contributor.author	Rodríguez-Galán, Olga
dc.contributor.author	Pelechano García, Vicente José
dc.contributor.author	Begley, Victoria
dc.contributor.author	Millán-Zambrano, G.
dc.contributor.author	Morillo-Huesca, Macarena
dc.contributor.author	Muñoz-Centeno, María de la Cruz
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Cruz, Jesús de la
dc.contributor.author	Holstege, Frank C. P.
dc.contributor.author	Chávez, Sebastián
dc.date.accessioned	2018-05-14T17:06:07Z
dc.date.available	2018-05-14T17:06:07Z
dc.date.issued	2017
dc.identifier.citation	Gómez-Herreros, Fernando Margaritis, Thanasis Rodríguez-Galán, Olga Pelechano García, Vicente José Begley, Victoria Millán-Zambrano, G. Morillo-Huesca, Macarena Muñoz-Centeno, María de la Cruz Pérez Ortín, José Enrique Cruz, Jesús de la Holstege, Frank C. P. Chávez, Sebastián 2017 The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation Nucleic Acids Research 45 16 9302 9318
dc.identifier.uri	http://hdl.handle.net/10550/66188
dc.description.abstract	Ribosome assembly requires the concerted expression of hundreds of genes, which are transcribed by all three nuclear RNA polymerases. Transcription elongation involves dynamic interactions between RNA polymerases and chromatin. We performed a synthetic lethal screening in Saccharomyces cerevisiae with a conditional allele of SPT6, which encodes one of the factors that facilitates this process. Some of these synthetic mutants corresponded to factors that facilitate pre-rRNA processing and ribosome biogenesis. We found that the in vivo depletion of one of these factors, Arb1, activated transcription elongation in the set of genes involved directly in ribosome assembly. Under these depletion conditions, Spt6 was physically targeted to the up-regulated genes, where it helped maintain their chromatin integrity and the synthesis of properly stable mRNAs. The mRNA profiles of a large set of ribosome biogenesis mutants confirmed the existence of a feedback regulatory network among ribosome assembly genes. The transcriptional response in this network depended on both the specific malfunction and the role of the regulated gene. In accordance with our screening, Spt6 positively contributed to the optimal operation of this global network. On the whole, this work uncovers a feedback control of ribosome biogenesis by fine-tuning transcription elongation in ribosome assembly factor-coding genes.
dc.language.iso	eng
dc.relation.ispartof	Nucleic Acids Research, 2017, vol. 45, num. 16, p. 9302-9318
dc.subject	Regulació genètica
dc.subject	Àcids nucleics
dc.subject	Cèl·lules
dc.title	The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation
dc.type	journal article	es_ES
dc.date.updated	2018-05-14T17:06:08Z
dc.identifier.doi	10.1093/nar/gkx529
dc.identifier.idgrec	118645
dc.rights.accessRights	open access	es_ES