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miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study

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miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study

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dc.contributor.author Micó Martínez, Pablo es
dc.contributor.author García Giménez, José Luis es
dc.contributor.author Seco Cervera, Marta es
dc.contributor.author López Roldán, Andrés es
dc.contributor.author Almiñana Pastor, Pedro J. es
dc.contributor.author Alpiste Illueca, Francisco M. es
dc.contributor.author Pallardó, Federico V. es
dc.date.accessioned 2018-05-28T10:45:23Z
dc.date.available 2018-05-28T10:45:23Z
dc.date.issued 2018 es
dc.identifier.citation Micó Martínez, Pablo ; Garcia Gimenez, Jose Luis ; Seco Cervera, Marta ; López Roldán, Andrés ; Almiñana Pastor, Pedro J. ; Alpiste Illueca, Francisco M. ; Pallardó Calatayud, Federico V. miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 23 3 2018: 8- es
dc.identifier.uri http://hdl.handle.net/10550/66443
dc.description.abstract The study and identification of new biomarkers for periodontal disease, such as microRNAs (miRNAs), may give us more information about the location and severity of the disease and will serve as a basis for treatment planning and disease-monitoring. miRNAs are a group of small RNAs which are involved in gene regulation by binding to their messenger RNA target (mRNA). In this pilot study, the procedure for purifying miRNAs from gingival crevicular fluid (GCF) was, for the first time, described. In addition, the concentration of miRNAs in GCF was analyzed and compared between patients with moderate or severe chronic periodontitis (CP) and healthy controls. GCF samples were collected from single-rooted teeth of patients with moderate or severe CP (n=9) and of healthy individuals (n=9). miRNAs were isolated from GCF using miRNeasy Serum/Plasma kit (Qiagen, CA. USA). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression of a series of miRNAs candidates that are related to bone metabolism. The significance of differences in miRNA levels between both groups was determined using Mann-Whitney U test. The results from this pilot study indicate that miRNAs can be isolated from GCF. Six different miRNAs were analyzed (miR-671, miR-122, miR-1306, miR-27a, miR-223, miR-1226), but only miR-1226 showed statically significant differences between the CP group and healthy controls (p<0.05). This miRNA was downregulated in patients with CP. Within the limitations of the present study, it may be concluded that miR-1226 can be a promising biomarker for periodontal disease, adding relevant information to common clinical parameters used for diagnosis and prognosis of periodontitis. es
dc.title miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.22329 es
dc.type.hasVersion VoR es_ES

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