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Stimulated and unstimulated saliva samples have significantly different bacterial profiles.

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Stimulated and unstimulated saliva samples have significantly different bacterial profiles.

dc.contributor.author	Gomar Vercher, Sonia
dc.contributor.author	Simón Soro, AureaA
dc.contributor.author	Montiel Company, José María
dc.contributor.author	Almerich Silla, José Manuel
dc.contributor.author	Mira, Alex
dc.date.accessioned	2018-06-28T07:07:35Z
dc.date.available	2018-06-28T07:07:35Z
dc.date.issued	2018
dc.identifier.citation	Gomar Vercher, Sonia Simón Soro, AureaA Montiel Company, José María Almerich Silla, José Manuel Mira, Alex 2018 Stimulated and unstimulated saliva samples have significantly different bacterial profiles. Plos One 13 6 e0198021
dc.identifier.uri	http://hdl.handle.net/10550/66804
dc.description.abstract	Epidemiological studies use saliva on a regular basis as a non-invasive and easy-to-take sample, which is assumed to be a microbial representative of the oral cavity ecosystem. However, comparative studies between different kinds of saliva samples normally used in microbial studies are scarce. The aim of the current study was to compare oral microbiota composition between two different saliva samples collected simultaneously: non-stimulated saliva with paper points and stimulated saliva collected after chewing paraffin gum. DNA was extracted from saliva samples of ten individuals, then analyzed by 16S rRNA pyrosequencing to describe bacterial diversity. The results demonstrate significant differences between the microbiota of these two kinds of saliva. Stimulated saliva was found to contain an estimated number of species over three times higher than unstimulated saliva. In addition, bacterial composition at the class and genus level was radically different between both types of samples. When compared to other oral niches, both types of saliva showed some similarity to tongue and buccal mucosa, but they do not correlate at all with the bacterial composition described in supra- or sub-gingival dental plaque, questioning their use in etiological and epidemiological studies of oral diseases of microbial origin.
dc.language.iso	cat
dc.relation.ispartof	Plos One, 2018, vol. 13, num. 6, p. e0198021
dc.subject	Epidemiologia
dc.subject	Estomatologia
dc.subject	Microbiologia
dc.subject	Boca
dc.title	Stimulated and unstimulated saliva samples have significantly different bacterial profiles.
dc.type	journal article	es_ES
dc.date.updated	2018-06-28T07:07:35Z
dc.identifier.doi	10.1371/journal.pone.0198021
dc.identifier.idgrec	126360
dc.rights.accessRights	open access	es_ES