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Differential preservation of endogenous human and microbial DNA in dental calculus and dentin

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Differential preservation of endogenous human and microbial DNA in dental calculus and dentin

dc.contributor.author	Mann, Allison E.
dc.contributor.author	Sabin, Susanna
dc.contributor.author	Ziesemer, Kirsten
dc.contributor.author	Vagene, Åshild J.
dc.contributor.author	Schroeder, Hannes
dc.contributor.author	Ozga, Andrew T.
dc.contributor.author	Sankaranarayanan, Krithivasan
dc.contributor.author	Hofman, Courtney A.
dc.contributor.author	Fellows Yates, James A.
dc.contributor.author	Salazar García, Domingo Carlos
dc.contributor.author	Frohlich, Bruno
dc.contributor.author	Aldenderfer, Mark
dc.contributor.author	Hoogland, Menno
dc.contributor.author	Read, Christopher
dc.contributor.author	Milner, George R.
dc.contributor.author	Stone, Anne C.
dc.contributor.author	Lewis, Cecil M.
dc.contributor.author	Krause, Johannes
dc.contributor.author	Hofman, Corinne
dc.contributor.author	Bos, Kirsten I.
dc.contributor.author	Wartinner, Christina
dc.date.accessioned	2018-07-10T11:20:05Z
dc.date.available	2018-07-10T11:20:05Z
dc.date.issued	2018
dc.identifier.citation	Mann, Allison E. Sabin, Susanna Ziesemer, Kirsten Vagene, Åshild J. Schroeder, Hannes Ozga, Andrew T. Sankaranarayanan, Krithivasan Hofman, Courtney A. Fellows Yates, James A. Salazar García, Domingo Carlos Frohlich, Bruno Aldenderfer, Mark Hoogland, Menno Read, Christopher Milner, George R. Stone, Anne C. Lewis, Cecil M. Krause, Johannes Hofman, Corinne Bos, Kirsten I. Wartinner, Christina 2018 Differential preservation of endogenous human and microbial DNA in dental calculus and dentin Scientific Reports 8 1 15
dc.identifier.uri	http://hdl.handle.net/10550/66948
dc.description.abstract	Dental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is microbial and originates from the oral microbiome; however, a small but consistent proportion of DNA (mean 0.08 ± 0.08%, range 0.007-0.47%) derives from the host genome. Host DNA content within dentin is variable (mean 13.70 ± 18.62%, range 0.003-70.14%), and for a subset of dentin samples (15.21%), oral bacteria contribute > 20% of total DNA. Human DNA in dental calculus is highly fragmented, and is consistently shorter than both microbial DNA in dental calculus and human DNA in paired dentin samples. Finally, we find that microbial DNA fragmentation patterns are associated with guanine cytosine (GC) content, but not aspects of cellular structure.
dc.language.iso	eng
dc.relation.ispartof	Scientific Reports, 2018, vol. 8, p. 1-15
dc.subject	Cromosomes humans
dc.title	Differential preservation of endogenous human and microbial DNA in dental calculus and dentin
dc.type	journal article	es_ES
dc.date.updated	2018-07-10T11:20:06Z
dc.identifier.doi	10.1038/s41598-018-28091-9
dc.identifier.idgrec	126447
dc.rights.accessRights	open access	es_ES