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Experimental study of the oriented immobilization of antibodies on photonic sensing structures by using protein A as an intermediate layer

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Experimental study of the oriented immobilization of antibodies on photonic sensing structures by using protein A as an intermediate layer

dc.contributor.author	Caroselli, Raffaele
dc.contributor.author	García Castelló, Javier
dc.contributor.author	Escorihuela Fuentes, Jorge
dc.contributor.author	Bañuls, María José
dc.contributor.author	Maquieira, Ángel
dc.contributor.author	García-Rupérez, Jaime
dc.date.accessioned	2018-09-07T14:28:12Z
dc.date.available	2018-09-07T14:28:12Z
dc.date.issued	2018
dc.identifier.citation	Caroselli, Raffaele García Castelló, Javier Escorihuela Fuentes, Jorge Bañuls, María José Maquieira, Ángel García-Rupérez, Jaime 2018 Experimental study of the oriented immobilization of antibodies on photonic sensing structures by using protein A as an intermediate layer Sensors 18 4
dc.identifier.uri	http://hdl.handle.net/10550/67434
dc.description.abstract	A proper antibody immobilization on a biosensor is a crucial step in order to obtain a high sensitivity to be able to detect low target analyte concentrations. In this paper, we present an experimental study of the immobilization process of antibodies as bioreceptors on a photonic ring resonator sensor. A protein A intermediate layer was created on the sensor surface in order to obtain an oriented immobilization of the antibodies, which enhances the interaction with the target antigens to be detected. The anti-bovine serum albumin (antiBSA)-bovine serum albumin (BSA) pair was used as a model for our study. An opto-fluidic setup was developed in order to flow the different reagents and, simultaneously, to monitor in real-time the spectral response of the photonic sensing structure. The antiBSA immobilization and the BSA detection, their repeatability, and specificity were studied in different conditions of the sensor surface. Finally, an experimental limit of detection for BSA recognition of only 1 ng/mL was obtained.
dc.language.iso	eng
dc.relation.ispartof	Sensors, 2018, vol. 18, num. 4
dc.subject	Òptica
dc.subject	Biotecnologia
dc.title	Experimental study of the oriented immobilization of antibodies on photonic sensing structures by using protein A as an intermediate layer
dc.type	journal article	es_ES
dc.date.updated	2018-09-07T14:28:13Z
dc.identifier.doi	10.3390/s18041012
dc.identifier.idgrec	126739
dc.rights.accessRights	open access	es_ES