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The 5' Untranslated Region of the EFG1 Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in Candida albicans

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The 5' Untranslated Region of the EFG1 Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in Candida albicans

dc.contributor.author	Desai, Prashant R.
dc.contributor.author	Lengeler, Klaus
dc.contributor.author	Kapitan, Mario
dc.contributor.author	Janßen, Silas Matthias
dc.contributor.author	Alepuz Martínez, Paula
dc.contributor.author	Jacobsen, Ilse D.
dc.contributor.author	Ernst, Joachim F.
dc.date.accessioned	2018-10-03T17:51:53Z
dc.date.available	2018-10-03T17:51:53Z
dc.date.issued	2018
dc.identifier.citation	Desai, Prashant R. Lengeler, Klaus Kapitan, Mario Janßen, Silas Matthias Alepuz Martínez, Paula Jacobsen, Ilse D. Ernst, Joachim F. 2018 The 5' Untranslated Region of the EFG1 Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in Candida albicans Msphere 3 4 e00280-18
dc.identifier.uri	http://hdl.handle.net/10550/67700
dc.description.abstract	Extensive 5' untranslated regions (UTR) are a hallmark of transcripts determining hyphal morphogenesis in Candida albicans The major transcripts of the EFG1 gene, which are responsible for cellular morphogenesis and metabolism, contain a 5' UTR of up to 1,170 nucleotides (nt). Deletion analyses of the 5' UTR revealed a 218-nt sequence that is required for production of the Efg1 protein and its functions in filamentation, without lowering the level and integrity of the EFG1 transcript. Polysomal analyses revealed that the 218-nt 5' UTR sequence is required for efficient translation of the Efg1 protein. Replacement of the EFG1 open reading frame (ORF) by the heterologous reporter gene CaCBGluc confirmed the positive regulatory importance of the identified 5' UTR sequence. In contrast to other reported transcripts containing extensive 5' UTR sequences, these results indicate the positive translational function of the 5' UTR sequence in the EFG1 transcript, which is observed in the context of the native EFG1 promoter. It is proposed that the 5' UTR recruits regulatory factors, possibly during emergence of the native transcript, which aid in translation of the EFG1 transcript. IMPORTANCE Many of the virulence traits that make Candida albicans an important human fungal pathogen are regulated on a transcriptional level. Here, we report an important regulatory contribution of translation, which is exerted by the extensive 5' untranslated regulatory sequence (5' UTR) of the transcript for the protein Efg1, which determines growth, metabolism, and filamentation in the fungus. The presence of the 5' UTR is required for efficient translation of Efg1, to promote filamentation. Because transcripts for many relevant regulators contain extensive 5' UTR sequences, it appears that the virulence of C. albicans depends on the combination of transcriptional and translational regulatory mechanisms.
dc.language.iso	eng
dc.relation.ispartof	Msphere, 2018, vol. 3, num. 4, p. e00280-18
dc.subject	Microbiologia
dc.title	The 5' Untranslated Region of the EFG1 Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in Candida albicans
dc.type	journal article	es_ES
dc.date.updated	2018-10-03T17:51:53Z
dc.identifier.doi	10.1128/mSphere.00280-18
dc.identifier.idgrec	127189
dc.rights.accessRights	open access	es_ES