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Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species

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Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species

dc.contributor.author	Bidart, Gonzalo N.
dc.contributor.author	Rodríguez Díaz, Jesús
dc.contributor.author	Palomino-Schätzlein, Martina
dc.contributor.author	Monedero García, Vicente
dc.contributor.author	Yebra Yebra, María Jesús
dc.date.accessioned	2018-11-16T14:35:00Z
dc.date.available	2018-11-16T14:35:00Z
dc.date.issued	2017
dc.identifier.citation	Bidart, Gonzalo N. Rodríguez Díaz, Jesús Palomino-Schätzlein, Martina Monedero García, Vicente Yebra Yebra, María Jesús 2017 Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species Applied Microbiology and Biotechnology 101 1 205 215
dc.identifier.uri	http://hdl.handle.net/10550/67948
dc.description.abstract	Lacto-N-biose (LNB) and galacto-N-biose (GNB) are major building blocks of free oligosaccharides and glycan moieties of glyco-complexes present in human milk and gastrointestinal mucosa. We have previously characterized the phospho-β-galactosidase GnbG from Lactobacillus casei BL23 that is involved in the metabolism of LNB and GNB. GnbG has been used here in transglycosylation reactions, and it showed the production of LNB and GNB with N-acetylglucosamine and N-acetylgalactosamine as acceptors, respectively. The reaction kinetics demonstrated that GnbG can convert 69 ± 4 and 71 ± 1 % of o-nitrophenyl-β-D-galactopyranoside into LNB and GNB, respectively. Those reactions were performed in a semi-preparative scale, and the synthesized disaccharides were purified. The maximum yield obtained for LNB was 10.7 ± 0.2 g/l and for GNB was 10.8 ± 0.3 g/l. NMR spectroscopy confirmed the molecular structures of both carbohydrates and the absence of reaction byproducts, which also supports that GnbG is specific for β1,3-glycosidic linkages. The purified sugars were subsequently tested for their potential prebiotic properties using Lactobacillus species. The results showed that LNB and GNB were fermented by the tested strains of L. casei, Lactobacillus rhamnosus (except L. rhamnosus strain ATCC 53103), Lactobacillus zeae, Lactobacillus gasseri, and Lactobacillus johnsonii. DNA hybridization experiments suggested that the metabolism of those disaccharides in 9 out of 10 L. casei strains, all L. rhamnosus strains and all L. zeae strains tested relies upon a phospho-β-galactosidase homologous to GnbG. The results presented here support the putative role of human milk oligosaccharides for selective enrichment of beneficial intestinal microbiota in breast-fed infants.
dc.language.iso	eng
dc.relation.ispartof	Applied Microbiology and Biotechnology, 2017, vol. 101, num. 1, p. 205-215
dc.subject	Microbiologia
dc.subject	Lactobacils
dc.title	Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species
dc.type	journal article	es_ES
dc.date.updated	2018-11-16T14:35:01Z
dc.identifier.doi	10.1007/s00253-016-7882-0
dc.identifier.idgrec	118843
dc.rights.accessRights	open access	es_ES