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Urinary exosome miR-146a is a potential marker of albuminuria in essential hypertension

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Urinary exosome miR-146a is a potential marker of albuminuria in essential hypertension

dc.contributor.author	Perez Hernandez, Javier
dc.contributor.author	Olivares, Dolores
dc.contributor.author	Forner, Maria J.
dc.contributor.author	Ortega, Ana
dc.contributor.author	Solaz, Elena
dc.contributor.author	Martinez, Fernando
dc.contributor.author	Chaves Martínez, Felipe Javier
dc.contributor.author	Redón i Más, Josep
dc.contributor.author	Cortes, Raquel
dc.date.accessioned	2019-02-07T11:39:00Z
dc.date.available	2019-02-07T11:39:00Z
dc.date.issued	2018
dc.identifier.citation	Perez Hernandez, Javier Olivares, Dolores Forner, Maria J. Ortega, Ana Solaz, Elena Martinez, Fernando Chaves Martinez, Felipe Javier Redón i Más, Josep Cortes, Raquel 2018 Urinary exosome miR-146a is a potential marker of albuminuria in essential hypertension Journal of Translational Medicine and Research 16 1 228
dc.identifier.uri	http://hdl.handle.net/10550/68893
dc.description.abstract	BACKGROUND: There is increasing interest in using extracellular vesicle-derived microRNAs (miRNAs) as biomarkers in renal dysfunction and injury. Preliminary evidence indicates that miRNAs regulate the progression of glomerular disease. Indeed, exosomes from the renal system have provided novel evidence in the clinical setting of albuminuria. Thus, the aim of this study was to quantify the urinary miRNAs present in exosome and microvesicles (MVs), and to assess their association with the presence of increased urinary albumin excretion in essential hypertension. METHODS: Exosomes were collected from urine specimens from a cohort of hypertensive patients with (n = 24) or without albuminuria (n = 28), and from 20 healthy volunteers as a control group. Urinary exosomes were phenotyped by Western blot, tunable resistive pulse sensing, and electronic microscopy. Expression of miR-146a and miR-335* was analysed by qRT-PCR and any associations between albuminuria and exosomal miRNAs were analysed. RESULTS: Urinary miRNAs are highly enriched in exosome subpopulations compared to MVs, both in patients with or without increased albuminuria (p < 0.001), but not in the control group. High albuminuria was associated with 2.5-fold less miR-146a in exosomes (p = 0.017), whereas miR-146a levels in MV did not change. In addition, exosome miR-146a levels were inversely associated with albuminuria (r = 0.65, p < 0.0001), and discriminated the presence of urinary albumin excretion presence [area under the curve = 0.80, 95% confidence interval: 0.66-0.95; p = 0.0013]. CONCLUSIONS: Our results indicate that miRNAs were enriched in the urinary exosome subpopulation in hypertensive patients and that low miR-146a expression in exosomes was associated with the presence of albuminuria. Thus, urinary exosome miR-146a may be a potentially useful tool for studying early renal injury in hypertension.
dc.language.iso	eng
dc.relation.ispartof	Journal of Translational Medicine and Research, 2018, vol. 16, num. 1, p. 228
dc.subject	Hipertensió pulmonar
dc.title	Urinary exosome miR-146a is a potential marker of albuminuria in essential hypertension
dc.type	journal article	es_ES
dc.date.updated	2019-02-07T11:39:00Z
dc.identifier.doi	10.1186/s12967-018-1604-6
dc.identifier.idgrec	129874
dc.rights.accessRights	open access	es_ES