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Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study

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Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study

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dc.contributor.author Foroughreyhani, Mohammad es
dc.contributor.author Salemmilani, Amin es
dc.contributor.author Mozafari, Aysan es
dc.contributor.author Soroush-Barhaghi, Mohammadhosien es
dc.date.accessioned 2019-03-11T08:31:58Z
dc.date.available 2019-03-11T08:31:58Z
dc.date.issued 2018 es
dc.identifier.citation Foroughreyhani, Mohammad ; Salemmilani, Amin ; Mozafari, Aysan ; Soroush-Barhaghi, Mohammadhosien. Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study. En: Journal of Clinical and Experimental Dentistry, 10 12 2018: 1223-1229 es
dc.identifier.uri http://hdl.handle.net/10550/69477
dc.description.abstract This in vitro study was mainly aimed to evaluate the effect of high-frequency alternating currents (AC) applied by an electronic apex locator (EAL) on the antibacterial properties of chlorhexidine (CHX) on E. faecalis biofilm. The root canals of 120 extracted human single-rooted teeth were prepared using Gates-Glidden drills and hand K-files. After contaminating the root canals with E. faecalis, they were incubated for 60 days. Then, the teeth were randomly divided into six experimental groups (n=20). Group 1, 2% CHX; group 2, normal saline (NS) with direct current (DC); group 3, normal saline (NS) with high-frequency alternating current (AC); group 4, 2% CHX with DC; group 5, 2% CHX with AC; group 6, control (normal saline). The samples were collected from the root canal walls of 16 teeth in each group and 1:10 serial dilutions were prepared and added to Muller-Hinton agar (MHA) plates and incubated at 37°C for 48 h. The longitudinal sections of the other 4 teeth used to observe under a scanning electron microscope (SEM). A classic colony counting technique was used for counting the vital E. faecalis bacteria in MHA. Two-way ANOVA was used for statistical analysis of the data. The level of significance was set at P<0.05. The electric current significantly changed the colony-forming units (CFU) values (P<0.001). According to pair-wise comparisons, the highest CFU difference was observed between the AC group and the group without electric current (P<0.001); furthermore, the difference between the DC group and the group without electric current was not significant (P=0.823). The highest bioelectric effect occurred with the use of high-frequency alternating electric current in the form of an apex locator with CHX as a canal irrigant. es
dc.title Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/jced.55369 es
dc.type.hasVersion VoR es_ES

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