NAGIOS: RODERIC FUNCIONANDO

Transmembrane but not soluble helices fold inside the ribosome tunnel

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Transmembrane but not soluble helices fold inside the ribosome tunnel

dc.contributor.author	Bañó Polo, Manuel
dc.contributor.author	Baeza Delgado, Carlos
dc.contributor.author	Tamborero Capilla, Silvia
dc.contributor.author	Hazel, Anthony
dc.contributor.author	Grau, Brayan
dc.contributor.author	Nilsson, IngMarie
dc.contributor.author	Whitley, Paul
dc.contributor.author	Gumbart, James C.
dc.contributor.author	Heijne, Gunnar von
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2019-05-07T13:10:27Z
dc.date.available	2019-05-07T13:10:27Z
dc.date.issued	2018
dc.identifier.citation	Bañó Polo, Manuel Baeza Delgado, Carlos Tamborero Capilla, Silvia Hazel, Anthony Grau, Brayan Nilsson, IngMarie Whitley, Paul Gumbart, James C. Heijne, Gunnar von Mingarro Muñoz, Ismael 2018 Transmembrane but not soluble helices fold inside the ribosome tunnel Nature Communications 9 5246 1 9
dc.identifier.uri	http://hdl.handle.net/10550/70100
dc.description.abstract	Integral membrane proteins are assembled into the ER membrane via a continuous ribosome-translocon channel. The hydrophobicity and thickness of the core of the membrane bilayer leads to the expectation that transmembrane (TM) segments minimize the cost of harbouring polar polypeptide backbones by adopting a regular pattern of hydrogen bonds to form α-helices before integration. Co-translational folding of nascent chains into an α-helical conformation in the ribosomal tunnel has been demonstrated previously, but the features governing this folding are not well understood. In particular, little is known about what features influence the propensity to acquire α-helical structure in the ribosome. Using in vitro translation of truncated nascent chains trapped within the ribosome tunnel and molecular dynamics simulations, we show that folding in the ribosome is attained for TM helices but not for soluble helices, presumably facilitating SRP (signal recognition particle) recognition and/or a favourable conformation for membrane integration upon translocon entry.
dc.language.iso	eng
dc.relation.ispartof	Nature Communications, 2018, vol. 9, num. 5246, p. 1-9
dc.subject	Proteïnes de membrana
dc.subject	Ribosomes
dc.title	Transmembrane but not soluble helices fold inside the ribosome tunnel
dc.type	journal article	es_ES
dc.date.updated	2019-05-07T13:10:27Z
dc.identifier.doi	10.1038/s41467-018-07554-7
dc.identifier.idgrec	129598
dc.rights.accessRights	open access	es_ES